氧化应激在实验性糖尿病神经病变中的作用  

THE ROLE OF OXIDATIVE STRESS IN EXPERIMENTAL DIABETIC NEUROPATHY

作　　者：王玲[1] 郑开俊[1] 董德勇[1] 孙悦[2] 王永明[3] 

机构地区：[1]天津医科大学生理教研室 [2]天津医科大学病理教研室, 天津300070 [3]天津医科大学毒理教研室,天津300070

出　　处：《中国应用生理学杂志》2008年第1期108-111,共4页Chinese Journal of Applied Physiology

摘　　要：目的:探讨氧化应激在糖尿病神经病变发生发展中的作用。方法:用链脲佐菌素诱导糖尿病大鼠模型。测定糖尿病不同时期坐骨神经中丙二醛(MDA)的水平、总超氧化物歧化酶(SOD)及Na+-K+依赖式ATP酶的活性。分析糖尿病大鼠坐骨神经中MDA水平与Na+-K+依赖式ATP酶活性的相关性。光镜下观察糖尿病不同时期坐骨神经的病理改变。结果:糖尿病1个月,3个月和6个月时MDA水平与对照组相比显著增高。糖尿病1个月时总SOD活性与对照组相比明显增高,3个月和6个月时活性明显降低。糖尿病3个月和6个月时Na+-K+依赖式ATP酶活性与对照组相比显著降低。糖尿病大鼠坐骨神经中MDA水平与Na+-K+依赖式ATP酶活性的相关性分析表明,两者呈显著的负相关。病理组织学观察表明,糖尿病3个月时坐骨神经出现病理改变,随着糖尿病病程的延长,病理改变加重。结论:糖尿病早期未出现神经病变时坐骨神经已存在明显的氧化应激,并且在神经病变发生后仍存在。氧化应激与坐骨神经中Na+-K+依赖式ATP酶的活性降低有关。因此,氧化应激在糖尿病神经病变的发生发展中起重要作用。Aim： To study the role of oxidative stress in the initiation and development of diabetic neuropathy,Methods： The diabetic rats were induced with streptozotocin（STZ ）. The malondialdehyde（MDA） level, total superoxide dismutase（SOD） and Na ＋ -K ＋ -ATPase activity were measured in the sciatic nerves at various stages of diabetes. The correlation of the MDA level and Na＋ -K＋ -ATPase activity was analyzed in diabetic rats. The pathological changes of sciatic nerve at diabetic various stages were examined by light microscopy. Results： The MDA level increased significantly in diabetic sciatic nerves as compared to controls at all time intervals. Total SOD activity increased significantly in diabetic sciatic nerves as compared to controls at one month of diabetes and progressively decreased at three/six months of diabetes. Na＋ -K＋ -ATPase activity progressively decreased at three/six months of diabetes. The correlation analysis indicated that the Na＋ -K＋ -ATPase activity was negative correlation with the MDA level in the diabetic rats. Histopathological study of the diabetic sciatic nerves showed that the pathological changes were observed at 3 months of diabetes, the changes were more serious as the diabetic duration was longer. Conclusion： Oxidative stress is found to occur during the early stages of STZ-induced diabetes （no neuropathy） and this state is maintained after initiation of neuropathy. The decreased Na＋ -K＋ -ATPase activity is associated with oxidative stress in the diabetic rats. Therefore, oxidative stress plays an important role in the initiation and development of diabetic neuropathy.

关 键 词：糖尿病 坐骨神经 丙二醛 超氧化物歧化酶 Na+-K+依赖式ATP酶 

分 类 号：R587.2[医药卫生—内分泌]