BMP2具有诱导C3H10T1/2细胞成脂肪分化的能力  

BMP2 Induces Adipogenic Differentiation of C3H10T1/2 Cells

作　　者：谢在春[1] 陈琼玉[2] 杨松海[2] 孙奋勇[2] 于永春[1] 

机构地区：[1]同济大学附属第十人民医院科教科,上海200072 [2]暨南大学生命科学技术学院生物工程研究所,广州510632

出　　处：《中国生物化学与分子生物学报》2008年第2期142-147,共6页Chinese Journal of Biochemistry and Molecular Biology

摘　　要：探讨骨形态发生蛋白2(BMP2)诱导鼠胚胎间充质干细胞C3H10T1/2成脂肪分化能力,为临床脂肪代谢疾病的治疗提供理论基础.培养多潜能的间充质干细胞C3H10T1/2,用20μg/mlBMP2对其诱导一定时间后,RT-PCR检测是否存在BMP信号通路中关键分子BMP受体BMPRI,BMPRⅡ及Smad1/5/8的表达.Western印迹检测Smad蛋白及MAPK信号通路中p38磷酸化水平变化,QRT-PCR检测成脂肪标志基因aP2以及成脂肪相关转录因子PPARγ,C/EBPα,C/EBPβ表达水平,同时用油红O染色,观测C3H10T1/2细胞成脂肪分化情况.经BMP2诱导后,C3H10T1/2细胞成脂肪分化标志(油红O染色)显著增加,Smad蛋白及p38磷酸化水平有所上升,同时成脂肪标志基因aP2以及成脂肪相关转录因子PPARγ,C/EBPα,C/EBPβ表达水平各有一定程度提高.BMP2具有诱导C3H10T1/2细胞成脂肪分化能力,其成脂肪分化呈现对BMP2作用的时间依赖性.To investigate the role of bone morphogenetic protein 2 （BMP2） in inducing the adipogenic differentiation from mesenchymal cell and exploit the potential applications for the treatment of lipometabolism dysfunctions, the mouse multi-potent C3H10T1/2 stem cells were treated with 20 μg/ml BMP2 for var/ous periods. The adipogenic process was characterized with Oil Red O staining in BMP2 treated cells. RT-PCR was employed to detect the existence of key molecules along the BMP signaling pathway, including BMPR Ⅰ , BMPR Ⅱ and Smadl/5/8. Western blot assays were used to examine the levels of phosphorylated Smad proteins and p38. The levels of an adipogenic marker, aP2 and transcription factors PPART, C/EBPa and C/EBPβ, were detected by quantitative RT-PCR. After the induction of BMP2, the Oil Red O demonstrated an obvious induction of adipogenic differentiation in C3H10T1/2 cells. The increased phosphorylation of Smad proteins and p38 were also observed. The levels of PPAR7, C/EBPα, C/EBPβ and aP2 were upregnlated at various degrees. The results demonstrated that BMP2 exhibited a potency to induce the adipogenic differentiation in C3H10T1/2 cells in a time-dependent manner.

关 键 词：骨形态发生蛋白 C3H10T1/2细胞 成脂分化 

分 类 号：Q291[生物学—细胞生物学] Q786