视黄酸挽救的视黄酸合成酶Raldh2基因敲除鼠E10.75胚胎后肢发育正常  

Raldh2 knock-out Embryos at E10.75 Rescued by Retinoic Acid Develop Normal Hindlimbs

作　　者：高元奇[1] 赵显玲[1] 

机构地区：[1]东北师范大学生命科学学院

出　　处：《中国生物化学与分子生物学报》2008年第2期148-152,共5页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金项目(No.30470903);留学回国人员启动基金项目~~

摘　　要：视黄酸合成酶Raldh2基因敲除鼠胚胎没有肢体的发育.在胚胎E6.75-E8.25期间,喂给怀孕母鼠含视黄酸(0.1mg/g食物)食物后,Raldh2基因敲除鼠E10.75胚胎后肢形态正常,前肢发育较小.原位杂交结果表明,决定肢体近-远端轴发育的标志基因(marker gene)Fgf8,决定前-后轴发育的标志基因Shh以及后肢发育特异性基因Tbx4和Pitx1在视黄酸挽救的Raldh2基因敲除鼠E10.75胚胎的后肢表达正常.上述结果提示,视黄酸可以挽救Raldh2基因敲除鼠E10.75胚胎后肢的正常发育.Retinaldehyde dehydrogenase-2 （RALDH2） catalyzes retinoic acid （RA） synthesis. Raldh2 knockout embryos have no limb buds development, but their embryos at E10.75 develop small forelimbs and morphological normal hindlimbs when pregnant female Raldh2 heterozygous knock-out mice were rescued by the food containing retinoic acid （0.1 mg/g food） from E6.75 to E8.25. The results of hybridization in situ showed that the expression of proximo-distal marker gene Fgf8, antero-posterior marker gene Shh and hindlimb specific marker gene Tbx4 and Pitxl was normal in the hindlimbs of RA rescued Raldh2 knock-out embryos at E10.75. This indicated that RA can rescue the normal hindlimbs development of Raldh2 knock-out embryo at E10.75.

关 键 词：视黄酸 视黄酸合成酶基因 肢体发育 挽救 小鼠 

分 类 号：Q81[生物学—生物工程]