近红外漫反射光谱法测定川西高原产沙棘中异鼠李素含量  

Determination of Isorhamnetin in Hippophae Rhamnoides Linn from West Sichuan Plateau Using Near Infrared Diffuse Reflectance Spectroscopy

作　　者：叶利明[1] 周旻[1] 张浩[1] 陈雏[1] 李章万[1] 陈聪[1] 王颜萍 

机构地区：[1]四川大学华西药学院,四川成都610041 [2]布鲁克光谱仪器公司,北京100081

出　　处：《光谱学与光谱分析》2008年第2期324-326,共3页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(30472153)资助

摘　　要：提出了用近红外光谱技术快速测定川西高原产沙棘中异鼠李素含量的方法。对异鼠李素含量在0.1%～0.8%范围内的川西高原产沙棘,采用SiO2拌样法,测定其在12000～4000cm-1的近红外吸收光谱,采用偏最小二乘算法建立了校正模型,比较了光谱不同预处理方法对校正结果的影响。当采用消除常数偏移量处理方法时能最有效地提取光谱中的有效信息,此时校正集相关系数(r2)为0.7398,校正集标准偏差(SEC)为0.107,预测集标准偏差(SEP)为0.073。该方法与传统方法相比更加快速简便。The objective of this study was to develop a method for the determination of isorhamnetin in Hippophae rhamnoides Linn from West Sichuan plateau using near infrared diffuse reflectance spectroscopy. Applying the method of mixing with SiO2, the near infrared spectra （NIS） with the range of 12 000-4 000 cm^-1 were recorded for the Hippophae rhamnoides Linn containing isorhamnetin with the content of 0. 1%-0. 8%. Calibration models were established using the PLS （partial least squares）. Different spectra pretreatments methods were compared. The study showed that spectral information can be extracted thoroughly by constant offset elimination （COE） pretreatments method with the correlation coefficient（rz ） of 0. 739 8, SEC of 0. 107 （standard deviation of the calibration sets） and SEP of 0. 073 （standard deviation of the prediction sets）. The results indicate that near infrared diffuse reflectance spectroscopy is more rapid and convenient than conventional methods.

关 键 词：近红外漫反射光谱 沙棘 SiO2拌样 异鼠李素 PLS 

分 类 号：O657.3[理学—分析化学] R927.2[理学—化学]