硫杂蒽酮稀土铕配合物与DNA相互作用的研究(Ⅱ)  

Study on the Interactions of Eu(Ⅲ) Complex of O-(Thioxanthone-[2]-yl)-Oxyacetic Acid with DNA

作　　者：杨武[1] 高琦宽[1] 芦小林[1] 吴锦绣[1] 

机构地区：[1]西北师范大学化学化工学院,甘肃兰州730070

出　　处：《光谱学与光谱分析》2008年第2期366-369,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(20475044);西北师范大学知识与科技创新工程项目(KJCX-01)资助

摘　　要：以荧光光谱,紫外吸收光谱和圆二色谱法研究了硫杂蒽酮稀土铕(Ⅲ)配合物与DNA的作用。结果表明,在pH7.10的模拟体液条件下,当DNA存在时,该化合物的紫外-可见吸收光谱随DNA浓度的增加表现出减色效应,使DNA的圆二色谱中的负峰发生较大变化,导致DNA的双螺旋结构变得松散。荧光光谱表明:该配合物的荧光强度在DNA存在时有较大的增加,表现出增色效应,同时使溴化乙锭(EB)-DNA体系的荧光强度降低,而溴化乙锭(EB)的存在也可使配合物-DNA体系的荧光强度降低。表明该配合物和EB与DNA有着强烈的竞争反应。据此推断,该配合物主要以嵌入方式与DNA作用。The interaction of the Eu（ Ⅲ ） complex of O-（thioxanthone-[2]-yl）-oxyacetic acid and calf thymus DNA was studied using fluorimetric, UV-Vis and CD（circular dichroism） methods. It was found that the ultraviolet absorption intensity of Eu（ Ⅲ） complex of O-（thioxanthone-[2]-yl）-oxyacetic acid decreased as the concentration of DNA increased under the physiological condition of pH 7. 10, and the compound remarkably influenced the negative peak in CD spectra of DNA when Eu（Ⅲ） complex of O-（thioxanthone-[2]-yl）-oxyacetic acid interacted with DNA. The emission intensity of the Eu（ Ⅲ ） complex evidently increased in the presence of DNA. The emission intensity of the DNA-EB system decreased as the concentration of the complex increasesd. And the presence of EB also led to the decrease in the emission intensity of the DNA-complex system. These changes observed here are often characteristic of intercalation. The above results strongly suggest that the complex can bind to DNA mainly by intercalation.

关 键 词：硫杂蒽酮稀土铕配合物 小牛胸腺DNA 溴化乙锭 

分 类 号：O614.3[理学—无机化学]