热凝固致良性前列腺增生组织在590～1064nm的吸收和散射特性的变化  

Thermal Coagulation of Human Benign Prostatic Hyperplasia Tissues Induced Changes in the Absorption and Scattering Properties in Spectral Range from 590 to 1064 nm in vitro

作　　者：魏华江[1] 邢达[1] 何博华[2] 吴荣海[3] 谷怀民[1] 巫国勇[4] 陈雪梅[5] 

机构地区：[1]华南师范大学激光生命科学研究所、暨激光生命科学教育部重点实验室,广东广州510631 [2]广东药学院临床医学系外科,广东广州510224 [3]广东省江门市中心医院泌尿外科,广东江门529071 [4]中山大学第一附属医院心胸外科,广东广州510080 [5]中山大学第一附属医院眼科,广东广州510080

出　　处：《光谱学与光谱分析》2008年第2期394-398,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(60378043;30470494);广东省自然科学基金项目(015012,04010394);广东省科技计划项目(2004B10401011)资助

摘　　要：研究了自然的和热凝固的良性前列腺增生(BPH)组织在590～1064nm光谱范围的光学特性及其差异,实验采用带积分球附件的分光光度计以及反向倍增法获取组织样品的吸收和散射特性参数。结果表明:热凝固导致BPH组织在590～1064nm光谱范围的吸收系数明显地减小的,自然的和热凝固的BPH组织的吸收系数都有一个峰值在990nm处,其值分别为0.438和0.416mm-1,自然的和热凝固的BPH组织的吸收系数的最大差异在1064nm,其值为86.79%,其最小差异在920nm,其值为4.74%。热凝固导致BPH组织在600～1064nm光谱范围的约化散射系数明显地增大,而在590nm处,热凝固导致BPH组织的约化散射系数却是明显地减小,自然的和热凝固的BPH组织的约化散射系数都有一个峰值在970nm处,其值分别为1.090和1.449mm-1,其另一个峰值都在1050nm处,其值分别为1.116和1.627mm-1,自然的和热凝固的BPH组织的约化散射系数的最大差异在1060nm,其值为47.73%,其最小差异在600nm,其值为4.86%。The optical properties and their differences of native and coagulated human benign prostatic hyperplasia （BPH） tissues were studied in the spectral range from 590 to 1 064 nm in vitro. The measurements were performed using a spectrophotometer with an integrating sphere attachment, and the absorption and scattering properties were assessed from these measurements using the inverse adding-doubling method. The results of measurement showed that the thermal coagulation of BPH tissues induced obviously the decrease in the absorption coefficients in the spectral range from 590 to 1 064 nm. The peaks in the absorption coefficients for native and coagulated BPH tissues were respectively 0. 438 and 0. 416 mm 1 corresponding to the same wavelength of 990 nm, the maximum difference in the absorption coefficients of native and coagulated BPH tissues is 86.79% at 1 064 nm, and the minimum difference is 4.74% at 920 nm. The thermal coagulation of BPH tissues induced an increase in the reduced scattering coefficients in the spectral range from 600 to 1 064 nm obviously, and induced a decrease in the reduced scattering coefficients at 590 nm obviously. The peaks in the reduced scattering coefficients for native and coagulated BPH tissues were respectively 1. 090 and 1. 449 mm^-1 corresponding to the same wavelength of 970 nm, and other peaks in the reduced scattering coefficients for native and coagulated BPH tissues were respectively 1. 116 and 1. 627 mm^-1 corresponding to the same wavelength of 1 050 nm, the maximum difference in the reduced scattering coefficients of native and coagulated BPH tissues is 47.73% at 1 064 nm, and the minimum difference is 4.86% at 600 nm.

关 键 词：热凝固 人良性前列腺增生组织 吸收和散射特性 积分球 

分 类 号：R318.51[医药卫生—生物医学工程]