吖啶黄反应光度法测定脱氧核糖核酸含量的研究  

Spectrophotometric Determination of Deoxyribonucleic Acid by Its Quenching Effect on Acridine Orange

作　　者：司文会[1] 訾言勤[2] 屠一锋[1] 

机构地区：[1]苏州大学化学化工学院 [2]淮北煤炭师范学院,安徽淮北235000

出　　处：《光谱学与光谱分析》2008年第2期412-414,共3页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(20675055)资助

摘　　要：以微量脱氧核糖核酸与吖啶黄的反应为基础,优化了实验条件。基于DNA与吖啶黄最大吸收波长444nm的吸光度有线性降低的关系,建立了测定微量DNA的新方法。在0～8.0μg.mL-1浓度呈现良好的线性关系,相关系数r为0.9998,检出限为0.12μg.mL-1。初步探讨了反应机理,认为DNA与吖啶黄通过嵌入作用生成了加合物。Based on the reaction of acridine orange with deoxyribonucleic acid（DNA）, and under the optimized condition, a novel method to determine the DNA was developed with the spectrophotometry. The absorbance of acridine orange at the maximum absorption wavelength of 444 nm responded to the concentration of DNA negatively with excellent linearity. It has an upper linear limit concentration of 8. 0 μg·mL^-1 and a detection limit of 0. 12 μg ·mL^-1 with the correlation coefficient of 0. 999 8. The method is simple, rapid and sensitive, and could be applied to sample assay satisfactorily. Finally, the reaction mechanism is discussed.

关 键 词：脱氧核糖核酸 吖啶黄 加合反应 含量测定 光度法 

分 类 号：O657.3[理学—分析化学]