石墨炉原子吸收法检测新生儿胎粪铅水平  

Determination of Meconium Lead Level of Newborn by Graphite Furnace Atomic Absorption Spectrometry

作　　者：李燕[1] 徐锡金[1] 刘俊晓[1] 郑良楷[1] 陈刚建[1] 陈松建[1] 霍霞[1] 

机构地区：[1]汕头大学医学院中心实验室,广东汕头515031

出　　处：《光谱学与光谱分析》2008年第2期447-449,共3页Spectroscopy and Spectral Analysis

基　　金：广东省自然科学基金项目(5008352);教育部留学回国人员科研启动基金项目资助

摘　　要：用硝酸消化新生儿胎粪铅含量的石墨炉原子吸收法(GFAAS)测定了144例新生儿胎粪铅的含量。0.3g胎粪于60℃烤箱烘烤过夜,80℃水浴下以HNO3和H2O2顺序消化1h后定容至2mL。用D2灯校正背景,样本基体匹配标准曲线法测定。实验组93例胎粪样本,铅平均值为1.934μg.g-1,标准差(SD)为1.551;对照组51例样本,平均值为1.012μg.g-1,标准差(SD)为1.084。实验组平均值明显高于对照组,两组均值比较做t检验p=0.000,p<0.05,两组均值在统计学上有显著差异。所建立的胎粪铅检测方法稳定,简便可行。To establish a method for the determination of lead in meconium from 144 samples of newborn through nitric acid digestion by means of graphite furnace atomic absorption spectrometry. Methods： after being baked for at least 12 h at 60 ℃, 0. 3 g of meconium was digested by nitric acid and hydrogen peroxide in turn at 80 ℃ under water-bath condition for lh and then metered to the whole volume of to 2 mL. After correcting the background with D2 lamp, specimen basal corpuscle matched standard curve was used to detect the lead content. Results： The mean lead content of 93 experimental samples was 1. 934 μg·g^-1 with the standard deviation （SD） of 1. 551, and that of the 51 control samples was 1. 012 μg ·g^-1, with the SD of 1. 084. There was a significant difference in lead levels of in meconnt between the experimental group and control group （p=0. 000）. Conclusion： The lead content of the experimental was significantly higher than that of the control group detected by this method. This method was stable and efficient.

关 键 词：胎粪 铅 原子吸收法 

分 类 号：O657.3[理学—分析化学]