A组人轮状病毒全基因组克隆和基因型分析  

Cloning of Full Genome and Genotyping of a Group A Human Rotavirus

作　　者：陈元鼎[1] 刘晓[1] 熊新宇[1] 曹志亮[1] 文喻玲[1] 赵庆欢[1] 余洋[1] 尹兴晓[1] 李传印[1] 范耀春[1] 

机构地区：[1]中国医学科学院北京协和医学院医学生物学研究所,昆明650118

出　　处：《中国生物工程杂志》2008年第2期25-31,共7页China Biotechnology

摘　　要：运用基因克隆和重组技术,从一急性胃肠炎患儿样品中克隆到一株轮状病毒(RV)全基因组cDNA。核苷酸序列测定结果表明,该株RV基因组11条RNA共含有18613个核苷酸(3302bp^751bp),编码5791个氨基酸。全基因组研究结果表明,该株RV(TB-Chen)为A组,II亚组,基因型为G2P[4]/NSP4[A]。这是首个A组人RV全基因组研究结果的报道,对研究和开发RV疫苗具有积极的意义。By means of genetic cloning and recombinant techniques, full genome cDNA sequences of rotavirus strain TB-Chen were isolated from an infantile hospitalized with acute gastroenteritis. Nucleotide sequences analyses showed that the full genome of strain TB-Chen contains 18613 nucleotides, encoding 5791 amino acids. Genotyping results showed that the strain TB-Chen belongs to genotype G2P[4]/NSp4 [A]. This is the first report on a full genome of Group A rotavirus in China, and has important significance for deep understanding structure and functions of rotaviruses and developing rotavirus vaccines.

关 键 词：A组人轮状病毒 全基因组 克隆 基因型分析 

分 类 号：Q785[生物学—分子生物学]