木质素降解菌的筛选及混合菌发酵降解秸秆的研究  

The Selection of Lignin-degrading Fungus and the Straw Fermentation by Mixed Strains

作　　者：黄茜[1] 黄凤洪[2] 江木兰[2] 万楚筠[2] 刘睿[2] 

机构地区：[1]华中农业大学食品科技学院,武汉430070 [2]中国农科院油料作物研究所,武汉430062

出　　处：《中国生物工程杂志》2008年第2期66-70,共5页China Biotechnology

基　　金：武汉市科技攻关资助项目(20062002063)

摘　　要：农作物秸秆是农业生产的副产品,也是一项重要的生物资源。其成分结构的特殊性所导致的难降解问题,一直是转化利用秸秆的难题。利用混合菌将秸秆纤维素转化为蛋白质、乙醇、乙酸、乳酸等研究是目前热点。通过马铃薯琼脂平板培养、马铃薯液体摇瓶培养和稻草秸秆固态发酵,从6株常见的食用白腐菌中筛选出了生长优势较强、产漆酶酶活高的平菇HF。为了让秸秆得到更好的降解和利用,采用平菇和康氏木霉二步混合发酵法;通过不同的组合方式,发现H6-T10组合得出的降解效果最好,其木质素降解率达到44.77%,纤维素降解率达到41.48%。The crops straw is an important biological resource. Because of its particular composition and structure, the hardly-degradation is always the problem of straw conversion and application. So Attention is paid to the desradafion and convertion of the straw into other useful materials. According to the PDA plate culture, shake-flask culture and solid-state fermentation with straw, the best strain which had growth advantage and yielded high activity lignin-degrading enzymes was chosen from 6 common white-rot funguses. In order to degrade and utilize the straw obviously, the two-step mixed fermentation by Pteurorus ostreatus and Trichoderma koningii was used. Through the different combination ways,H6-T10 degradation rate reached 44.77% group can get the best straw degradation result. The lignin and the cellulose degradation rate was 41.48%.

关 键 词：秸秆降解 混合发酵 白腐真菌 木质素 纤维素 

分 类 号：Q819[生物学—生物工程]