侵染落葵的黄瓜花叶病毒分离物CP基因序列分析  

Sequence Analysis of Coat Protein Gene of Cucumber Mosaic Virus Isolate Infecting Basella rubra L.

作　　者：牛立霞[1] 牛胜鸟[1] 王健华[1] 刘志昕[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所热带作物生物技术国家重点实验室,海口571101

出　　处：《中国生物工程杂志》2008年第2期96-100,共5页China Biotechnology

基　　金：国家"十一五"科技支撑计划资助项目(2007BAD48B01)

摘　　要：通过间接酶联免疫法（ID-ELISA）检测到染病落葵病样中存在黄瓜花叶病毒（cucumber mosaic virus，CMV）。从病叶中提取总RNA，用RT-PCR方法扩增得到657bp的CMVCP基因片段，将扩增产物与T载体连接并进行测序。用DNAMAN将得到的CP基因序列与GenBank收录的黄瓜花叶病毒两亚组部分株系或分离物的CP基因序列进行比较，结果表明该CP基因与CMV亚组I、亚组Ⅱ之间的核苷酸序列同源性分别为91．17％～95．43％和75．30％～75．76％，推导氨基酸序列同源性分别为95．41％～97．71％和81．28％～81．74％，表明CMV．Ba与亚组I同源关系密切。Cucumber mosaic virus was detected from infected Basella rubra L. with the indirect enzymelinked immunosorbent assay. Total RNA was extracted from infected leaves and the cDNAs of coat protein gene of CMV-Ba were obtained by RT-PCR. The amplified cDNA fragments were then cloned into pMD 18-T vector and sequenced, the result showed that the CP gene was 657 nucleotides in length. This sequence was aligned with the obtained CP gene and some CMV strains or isolates of subgroup I and subgroup 11 in GenBank using DNA MAN software. The results showed that CMV -Ba shared 90.9% - 93.8% and 76. 1% - 76.9% identity with the known CP genes of subgroup I and 11 respectively in nucleotide level,on the other hand, amino acids deduced from CMV-Ba CP gene shared 92.7% - 97.7% and 72.4% - 78. 1% identity with the known CP protein of subgroup I and Ⅱ ,respectively. This suggested that CMV-Ba CP gene belongs to CMV subgroup I .

关 键 词：落葵 黄瓜花叶病毒 CP基因 序列分析 

分 类 号：Q78[生物学—分子生物学]