糖原合成酶激酶3抑制剂降低胰岛β细胞凋亡  

Protection of INS-1 cells against free fatty acid-induced apoptosis by inhibiting the activity of glycogen synthase kinase-3

作　　者：吴薇[1] 罗小平[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院儿科学系,武汉430030

出　　处：《中华内分泌代谢杂志》2008年第1期48-49,共2页Chinese Journal of Endocrinology and Metabolism

基　　金：国家自然科学基金资助项目（30672262）;教育部高等学校博士学科点专项科研基金资助项目（20060487062）

摘　　要：用油酸诱导胰岛INS-1细胞凋亡，分别用流式细胞仪检测INS-1细胞的凋亡率，并用Western印迹检测糖原合成酶激酶3（GSK-3）磷酸化的水平，GSK-3抑制剂氯化锂（LiCl）干预后，油酸诱导的INS-1细胞凋亡率降低，GSK-3磷酸化增加，提示通过抑制GSK-3活性可降低胰岛β细胞凋亡率。Apoptosis in INS-1 cells was induced by oleic acid. The propidium iodide staining analysis by flow cytometry was applied to evaluate apoptosis, and glycogen synthase kinese-3 （GSK-3） phosphorylation were analyzed by Western blot. While being treated with LiCl （ an inhibitor of GSK-3 ）, apoptosis of INS-1 cells induced by oleic acid was lessened and phosphorylation of GSK-3 increased remarkably. Hence, inhibition of GSK-3 activity can effectively reduce the apoptosis of INS-1 ceils induced by oleic acid.

关 键 词：糖原合成酶激酶3 凋亡 油酸 

分 类 号：R723.14[医药卫生—儿科] R725.8[医药卫生—临床医学]