He-Ne激光和增强UV-B辐射对小麦幼苗蛋白质代谢的影响  

Effect of He-Ne Laser and Enhanced Ultraviolet-B Radiation on Protein Metabolism of Wheat Seedlings

作　　者：王小花[1] 刘海峰[2] 韩榕[1] 

机构地区：[1]山西师范大学生命科学学院细胞工程研究所,山西临汾041004 [2]南京师范大学生命科学学院江苏省分子医学生物技术重点实验室,南京210046

出　　处：《西北植物学报》2008年第1期103-108,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金项目(30671061);山西省自然科学基金项目(20041101)

摘　　要：采用He-Ne激光(5 mW.mm-2)和增强UV-B(10.08 kJ.m-2.d-1)辐照‘晋麦8号’小麦幼苗,5 d后测定各处理组小麦叶片的蛋白酶、转氨酶活性以及可溶性蛋白质含量与组成的变化。结果显示,增强UV-B辐射使小麦叶片蛋白酶活性极显著升高,转氨酶活性降低,可溶性蛋白质含量极显著下降,蛋白质谱带增加;单独He-Ne激光处理使蛋白酶活性下降,转氨酶活性升高,可溶性蛋白质含量增加,对蛋白质条带影响不明显;与单独UV-B辐射相比,经He-Ne激光辐照和UV-B辐射复合处理后,蛋白酶的活性明显降低,转氨酶的活性增加,可溶性蛋白质含量增加,并且使UV-B辐射诱导出的新带减弱或消失。研究发现,增强UV-B辐射能减弱小麦幼苗蛋白代谢中正常基因的表达,但又激活了一些抗性基因的表达而诱导产生新的胁迫蛋白;一定剂量的He-Ne激光辐照可解除UV-B对小麦幼苗正常基因表达的抑制而使其蛋白质代谢加强。When the seedlings of wheat ＇Jinmai 87 were exposed to both He-Ne laser irradiation （5 mW · mm^-2）and enhanced UV-B radiated radiation （10.08 kJ · m^-2 · d^-1） ,the activities of protease and aminotransferase, the content and composition of soluble protein were changed after five days. The results showed that the wheat seedlings with enhanced UV-B radiation treatment increased the protease activity and decreased the aminotransferase activity. Meanwhile,the content of soluble protein decreased. However, the opposite results were obtained with He-Ne Laser treatment. Compared with the control, the influence to wheat seedlings induced by enhanced UV-B radiation can be alleviated by He-Ne laser irradiation with decrease of protease activity,increase of aminotransferase activity and increase of the content of soluble protein. The new bands induced by enhanced UV-B radiation were weakened or disappeared because of He-Ne laser irradiation. In conclusion, our study found that some resistant genes were activated and some new threatening proteins were induced. The suppression of regular gene expression by UV-B radiation was re- lieved and the protein metabolism was enhanced after He-Ne laser irradiation.

关 键 词：HE-NE激光 UV—B 小麦 蛋白质代谢 

分 类 号：Q945.78[生物学—植物学]