乙型肝炎病毒前C/BCP区基因变异对特异性细胞毒性T淋巴细胞免疫应答的影响  

The influences of hepatitis B virus precore and basic core promoter region mutations on the immune responses of specific cytotoxic T lymphocytes

作　　者：杨小娟[1] 吴国荣[2] 裴豪[1] 钱金娟[1] 季瑞云[1] 

机构地区：[1]无锡市传染病医院检验科,214005 [2]无锡市第一人民医院实验室

出　　处：《中华传染病杂志》2007年第12期723-725,共3页Chinese Journal of Infectious Diseases

摘　　要：目的研究HBV前C/BCP区基因变异对慢性乙型肝炎(CHB)患者特异性细胞毒性T淋巴细胞(CTL)免疫应答的影响。方法采用HBV核心抗原表位肽core18-27,流式细胞术胞内细胞因子(CFC)分析法检测CHB患者外周血单个核细胞(PBMC)中的特异性CTL,对扩增产物进行测序分析。结果54例CHB患者中G1896A突变毒株21例,占38.9%;1762/1764位核苷酸联合突变26例。占48.1%;3位点同时突变毒株13例,占24.1%。3种变异株体外HBV核心抗原表位肽core18-27刺激后,特异性CTL水平[(0.41±0.09)%、(0.36±0.08)%、(0.48±0.08)%]显著高于野毒株[(0.11±0.06)%,P<0.05]。结论G1896A变异及1762/1764位核苷酸联合突变能显著增强特异性CTL水平,在CHB患者病情活动过程中起重要作用。Objective To investigate the influences of mutation at precore and basic core promoter（BCP） region in hepatitis B virus（HBV） on the immune response of specific cytotoxic T lymphocytes（CTL） in patients with chronic hepatitis B（CHB）. Methods The number of specific CTL in peripheral blood mononuclear（PBMC） of CHB patients were tested by cytokine flow cytometry（CFC） and HBV core18-27 peptide. HBV precore and BCP fragments were directly sequenced. Results Twenty-one（38.9%） samples were HBV precore G1896A mutation. Twenty-six（48. 1%） samples were BCP region 1762/1764 combined mutation. Thirteen（24. 1%） stains were three sites mutated simultaneously. Stimulated with HBV core 18-27 in vitro, the specific CTL level was significantly higher in the patients with G1896A mutation and BCP region mutation [（0. 41± 0. 09）%, （0.36 ± 0.08） %, （0.48 ± 0.08） %, respectively] than those without mutation[（0. 11 ± 0.06） %, P 〈 0.05]. Conclusion Specific CTL level is significantly increased due to G1896A mutation and BCP region mutation, which can play an important role in the pathogenesis of CHB.

关 键 词：基因 病毒 变异(遗传学) 肝炎 乙型 慢性 T淋巴细胞 细胞毒性 

分 类 号：R512.6[医药卫生—内科学] R392[医药卫生—临床医学]