Hedgehog信号通路特异性抑制剂cyclopamine对HaCaT细胞白介素8分泌的影响  

Influence of cyclopamine, a specific inhibitor of Hedgehog pathway, on the secretion of interleukin 8 in HaCaT cells

作　　者：周扬[1] 刘海燕[1] 刘玉峰[1] 李承新[1] 

机构地区：[1]第四军医大学全军皮肤病研究所,陕西西安710032

出　　处：《临床皮肤科杂志》2008年第3期157-159,共3页Journal of Clinical Dermatology

基　　金：国家自然科学基金资助项目(30572099);教育部新世纪优秀人才资助计划资助项目

摘　　要：目的:探讨Hedgehog信号通路特异性抑制剂cyclopamine对人正常角质形成细胞HaCaT细胞白介素(IL)-8分泌的影响。方法:在HaCaT细胞培养基中加入cyclopamine,采用反转录(RT)-PCR和ELISA方法检测HaCaT细胞在cyclopamine处理后不同时段IL-8 mRNA及其分泌的变化。结果:HaCaT细胞在cyclopamine处理后IL-8的mRNA表达及其分泌均减少(P<0.05)。结论:Hedgehog信号通路特异性抑制剂cyclopamine可抑制HaCaT细胞分泌IL-8。Objective： To investigate the influence of cyclopamine, a specific inhibitor of Hedgehog pathway, on the secretion of interleukin 8 （IL-8） in HaCaT cells. Methods： RT-PCR and ELISA were employed to measure the secretion of IL-8 in HaCaT cells at different time after treatment with cyclopamine. Results： IL-8 mRNA expression was significantly inhibited in HaCaT cells treated with cyclopamine, and IL-8 secretion was also reduced （P 〈 0.05）. Conclusion： Cyclopamine, a specific inhibitor of Hedgehog pathway, can suppress the IL-8 secretion of HaCaT keratinocytes.

关 键 词：HEDGEHOG信号通路 CYCLOPAMINE 角质形成细胞 ELISA RT-PCR 

分 类 号：R285.5[医药卫生—中药学]