10种冬青属植物遗传多样性RAPD和AFLPs分析  

STUDIES OF GENETIC DIVERSITY AMONG 10 SPECIES OF ILEX BASED ON RAPD AND AFLPs

作　　者：钱永生[1] 王慧中[1] 施农农[1] 赵艳[2] 黎念林[3] 胡中[3] 

机构地区：[1]杭州师范大学生物化学与分子生物学杭州市重点实验室,杭州310018 [2]浙江工商大学,生物工程系,杭州310018 [3]杭州植物园环境绿化工程部,杭州310013

出　　处：《分子细胞生物学报》2008年第1期35-43,共9页Journal of Molecular Cell Biology

基　　金：浙江省自然科学基金(301406);浙江省"151"和杭州市"131"人才基金资助项目~~

摘　　要：采用RAPD和AFLP技术,对10种冬青属植物基因组进行DNA片段扩增,以研究该属种间遗传多样性。结果表明:在RAPD分析中,通过对100种10个碱基随机引物的筛选,发现11种引物能得到多态性较高扩增产物,11种引物共扩增出301条多态性条带,多态率为98.63%。在AFLP分析中,3对选择性引物组合均扩增出了丰富的多态性片段。利用RAPD和AFLP技术分析,结果按UPGMA类平均法进行聚类,聚类结果显示冬青和代茶冬青,木姜冬青和浙江冬青以及光枝刺缘冬青与毛枝三花冬青之间的亲缘关系最近。The techniques of random amplified polymorphic DNA （RAPD） and amplified fragment length polymorphisms （AFLPs） were used to amplify the genomic DNA of 10 species of Ilex, so as to reveal the polymorphism in different species of Ilex. In RAPD analysis, a total of 301 lines were amplified with 11 kinds of primers selected from 100 kinds of 10 bp random primers, and the polymorphism was 98.63%. In AFLPs analysis,a huge diversity was showed by 3 primers combinations carried out on these species. Both RAPD and AFLPs analysis were clustered by UPGMA and the results showed that the genetic distances were the closest between I. purpurea and I. vomitoria A it, I. Litseaefolia and I. zhejiangensis, I. hylonoma var. glabra and I. triflora var. kanehirai.

关 键 词：冬青属 RAPD AFLP 遗传多样性 

分 类 号：Q943[生物学—植物学]