共振光散射光谱研究马歇尔红与牛血清白蛋白的作用及其分析应用  

Study of the Interaction of Bovine Serum Albumin(BSA) with Marshall Red and Its Analytical Application by Resonance Light Scattering Technique

作　　者：胡文康[1] 李原芳[1] 龙云飞[1] 

机构地区：[1]西南大学化学化工学院

出　　处：《西南大学学报（自然科学版）》2008年第1期7-11,共5页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(30570465)

摘　　要：研究了马歇尔红（Marshall red R,MR）与牛血清蛋白（BSA）作用的共振光散射（RLS）光谱特征.考察了各种影响因素并计算出了BSA与MR的结合比（质量比）为3.2.研究表明,在优化条件下,体系的RLS强度与蛋白质浓度在一定范围内具有良好的线性关系,据此建立了一种蛋白质测定的新方法.在最佳实验条件下,BSA的线性范围是0.03～6.0μg/mL,最低检出限为0.9 ng/mL.该方法用于合成样品及尿液的测定,结果满意.The interaction of bovine serum albumin （BSA） with MR was studied by resonance light scattering （RLS）technique and UV-vis spectra. At pH 2.36, a new method for the determination of proteins based on the enhancement of RLS was developed. Under the optimum conditions, the linear ranges of the calibration graphs were 0. 03-6.0μg/mL for proteins and the corresponding determination limit was 0. 9 ng/mL. Satisfactory results were obtained when this method was applied in the analysis of samples of foreign substances and urine.

关 键 词：马歇尔红 蛋白质 共振光散射 

分 类 号：O657.3[理学—分析化学] Q512.1[理学—化学]