用吴茱萸乙醇提取物对斜纹夜蛾的生物活性进行研究  

Study on the Bioactivity of Evodia rutaecarpa(Juss.) Benth. Ethanol Extracts Against Spodoptera litura Fab.

作　　者：蒋春先[1] 熊忠梅[1] 李庆[1] 王海建[1] 杨群芳[1] 

机构地区：[1]四川农业大学农学院,四川雅安625014

出　　处：《西南大学学报（自然科学版）》2008年第2期105-108,共4页Journal of Southwest University(Natural Science Edition)

基　　金：四川省教育厅资助项目(2003A002)

摘　　要：室内测定了吴茱萸(Evodia rutaecarpa(Juss.)Benth.)乙醇提取物对斜纹夜蛾(Spodoptera lituraFab.)幼虫的生物活性.结果表明,吴茱萸乙醇提取物对斜纹夜蛾幼虫有较强的拒食活性和生长发育抑制作用,提取物浓度越高,效果越明显.叶碟法测定吴茱萸乙醇提取物对斜纹夜蛾的选择性和非选择性拒食中浓度(AFC50)分别为0.657 3 mg/mL和1.178 mg/mL.人工饲料法测定结果表明吴茱萸乙醇提取物对斜纹夜蛾幼虫的拒食活性随提取物浓度增加、作用时间延长而增加.另外吴茱萸乙醇提取物能显著抑制斜纹夜蛾幼虫生长发育,引起发育历期增加,化蛹率减少和蛹质量降低.The bioactivity of Evodia rutaecarpa （Juss.） Benth. ethanol extracts against Spodoptera litura Fab. was investigated in laboratory. The results showed that the extracts had very strong antifeedant activity for the pest and inhibition of its development. The bioactivity of E. rutaecarpa （Juss.） Benth. ethanol extracts against S. litura Fab. increased with the concentration of the extracts. The choice and non-choice AFC50 was 0. 657 3 mg/mL and 1. 178 mg/mL, respectively, when the extracts were applied on leaf discs. When applied by incorporation into artificial diet, Evodia rutaecarpa （Juss.） Benth. ethanol extracts showed increasing bioactivity against S. litura Fab. as the concentration of extracts increased and as the time prolonged. The extracts also prolonged larval period, reduced pupal rate and decreased pupal weight.

关 键 词：吴茱萸 斜纹夜蛾 生物活性 拒食作用 生长发育抑制作用 

分 类 号：Q949.752.7[生物学—植物学]