铅对大鼠海马脑片谷氨酸递质体外释放的影响  

Effects of Lead on the Release of Glutamate Neurotransmitter in Rat Hippocampal Slices

作　　者：张亨山[1] 赵西龙[1] 宋丽华[1] 秦钰慧[1] 

机构地区：[1]中国预防医学科学院环境卫生监测所毒理室

出　　处：《中华预防医学杂志》1997年第5期285-287,共3页Chinese Journal of Preventive Medicine

摘　　要：为了探讨铅损害学习记忆功能的神经生物学机制，采用大鼠海马脑片观察铅在体外对谷氨酸递质释放的影响。结果显示，３．１～５０．０μｍｏｌ／Ｌ铅使海马脑片无钙状态下的３Ｈ－ＤＬ－谷氨酸自发性释放量增加２３．２％～６６．２％，并有剂量－效应关系。但在含钙介质中，当铅染毒剂量从１．０μｍｏｌ／Ｌ增加为５０．０μｍｏｌ／Ｌ时，３Ｈ－ＤＬ－谷氨酸的自发性释放量减少２２．６％～５５．３％，而高钾去极化释放量增加８９．３％～３３２．１％，而且均存在剂量－效应关系。表明铅对谷氨酸递质自发性和去极化释放过程均有干扰作用。Effects of lead on the release of glutamate neurotransmitter were observed in rat hippocampal slices in vitro to study its neurobiological mechanism of lead induced impairment in learning and memory function. Results showed that exposure to 3.1 to 50.0 μmol/L of lead could cause a dose dependent increase in spontaneous release of 3H DL glutamic acid in hippocampal slices by 23.2% to 66 2% in the absence of calcium. However, in the presence of calcium, spontaneous release of 3H DL glutamic acid decreased by 22.6% to 55.3% with increase of exposure to lead from 1.0 to 50.0 μmol/L, and the release induced by potassium increased by 89.3% to 332.1% with a dose response relationship. It indicated that exposure to lead could interfere with spontaneous and induced release of glutamate transmitter.

关 键 词：铅 海马 谷氨酸 体外释放 学习 记忆 损害 

分 类 号：R995[医药卫生—毒理学] R363.16[医药卫生—药学]