无机砷对人皮肤成纤维细胞增殖毒性的实验观察  

Toxic Effects of Proliferation on Skin Fibroblasts Induced by Inorganic Arsenic

作　　者：江兴林[1] 刘立亚[1] 黄雪梅[1] 胡昌军[1] 

机构地区：[1]怀化医学高等专科学校医学检验系,湖南怀化418000

出　　处：《实用预防医学》2008年第1期25-26,共2页Practical Preventive Medicine

基　　金：湖南省(教育厅)高等学校科学研究项目(05C018)

摘　　要：目的观察亚砷酸钠诱导体外培养的人皮肤成纤维细胞的作用,探讨砷性皮肤损伤的分子机制。方法通过直接细胞计数法和噻唑蓝(MTT)还原法检测亚砷酸钠对皮肤成纤维细胞生长的影响。结果与对照组比较,皮肤成纤维细胞经不同剂量的亚砷酸钠诱导后,吸光度值均有改变,存在剂量-效应关系(P<0.01)。0.5～5.0μmol/L浓度的亚砷酸钠对皮肤成纤维细胞有明显的增殖作用,而10μmol/L浓度的亚砷酸钠产生明显的毒性作用。结论低浓度NaAsO2、NaAsO2刺激人皮肤成纤维细胞增殖,高浓度具有细胞毒性。Objective To study the toxic effects of inorganic arsenic on skin fibroblasts proliferation, and to explore the molecular mechanism of sodium arsenite for its role in skin lesions from arsenic contamination. Methods Cell number counting, MTT reduction assay, transmission electron microscope were performed in skin fibroblasts,and sodium arsenite was used as tester for inorganic arsenics. Results With different concentration of sodium arsenite 0.5 ～ 5.0 μmol/L, the skin fibroblasts were markedly stimulated, and a dose effect relationship was observed. At the concentration of 10.0 μmol/L, however,sodium arsenite induced injury of skin fibroblasts. Conclusions The proliferation of the skin fibroblasts is regulated in a doublephase manner by sodium arsenite. Low concentration induces stimulation effect, but high concentration inhibits the cell proliferation.

关 键 词：亚砷酸钠 皮肤成纤维细胞 增殖 

分 类 号：R995[医药卫生—毒理学]