宁夏苦豆子根的高效液相色谱指纹图谱研究  

HPLC Fingerprint Spectrum of Sophora alopecuroides L.

作　　者：杨敏丽[1] 陈文娟[1] 李鹏[1] 

机构地区：[1]宁夏大学能源化工自治区重点实验室,宁夏银川750021

出　　处：《时珍国医国药》2008年第2期268-270,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金项目(No.20565003);宁夏自治区科技攻关项目(No.06273)

摘　　要：目的建立宁夏苦豆子根的指纹图谱分析方法。方法采用高效液相色谱法以ZORBAX SB-C18(250 mm×6 mm,5μm)为色谱柱;流动相为乙腈∶0.1%H3PO4+0.1%(C2H5)3N,梯度洗脱;流速1.0 ml.min-1;检测波长215 nm;柱温30℃。结果用梯度洗脱得到的色谱图各色谱峰分离效果较好,达到指纹图谱要求。结论为更好地控制宁夏苦豆子的内在质量提供了可靠的分析方法。Objective To establish a fingerprint analysis method of roots of Sophora alopecuroides L. Methods The fingerprint was performed by HPLC. ZORBAX SB - C18 column was used;the mobile phase was composed of CH3CN , 0.1% H3PO4 and 0.1% （C2H5 ）3 N with gradient elution ;flow rate was 1.0 ml·min^-1 ;the detection wavelength was 215 nm ;the column temperature was 30℃. Results HPLC fingerprint of Sophora alopecuroides L. was obtained. Conclusion A reliable method is provided for the quality conorol of Sophora alopecuroides L.

关 键 词：苦豆子 指纹图谱 高效液相色谱 

分 类 号：R282.5[医药卫生—中药学]