大蓟中芸香苷超声提取工艺的优化  

Optimization of Ultrasonic Extraction of Rutin in Cirsium japonicum DC

作　　者：翁涛[1] 邵宏[2] 伍宇婷[2] 

机构地区：[1]武汉大学药学院,湖北武汉430072 [2]武汉生物工程学院,湖北武汉430415

出　　处：《时珍国医国药》2008年第2期435-436,共2页Lishizhen Medicine and Materia Medica Research

摘　　要：目的优化大蓟中总黄酮的提取工艺;考察药材粉碎度、提取时间、料液比的最佳条件。方法采用超声提取法、正交实验法,以分光光度法测定提取物中总黄酮的含量。结果确定了最佳工艺条件,40℃下采用80%甲醇超声提取,料液比1∶10,粉碎度60目,超声提取时间3h。结论用上述方法可优化提取工艺。Objective To explore an optimum extraction technology of the total flavones in the Cirsium japonicum DC. and to investigate the optimum condition of the particle size of Cirsium japonicum DC. , extraction time, ratio of liquor to material Methods The total flavones in the Cirsium japonicum DC. was extracted by ultrasonic method, the conditions were optimized by the orthogonal experiment and the content of total flavones was determined by UV. Results The optimum extraction was determined as follows： the particle size 60 screen mesh, ratio of liquor to material was 1： 10 at 40℃ for 3 hours with 80% ethanol Conclusion Extraction technology can be optimized by the above -mentioned methods.

关 键 词：大蓟 超声提取 提取工艺 正交实验 

分 类 号：R284.2[医药卫生—中药学]