高效薄层色谱法检测黄藤素中掺杂的黄连素  

HPTLC Determination of Synthetic Berberine Mixed in Fibrauretinum as Adulterant

作　　者：郑萍[1] 谢笑天[1] 李国宝[1] 范亚刚[2] 

机构地区：[1]云南师范大学化学系,昆明650092 [2]云南省药品检验所

出　　处：《中草药》1997年第9期527-529,共3页Chinese Traditional and Herbal Drugs

摘　　要：采用高效薄层预制板，用双向展开薄层色谱法分离检测黄藤素中掺杂的人工合成黄连素，在波长λ＝365nm下荧光定位，用薄层扫描法测定其含量。此法还可测定黄藤素中巴马汀与药根碱的含量，方法简便、快速、准确、重现性好。A two dimensional thin layer chromatographic method was established to determine the amount of syn thetic berberine mixed in fibrauretinum as adulterant by HPTLC plate. Flurecence localization was at λ=365 nm. The method has been applied for the quantitative determination of palmatine and jatrorrhizine in fibrau retinum and proved to be simple,accurate and reproducible

关 键 词：HPTLC 黄藤素 黄连素 薄层色谱法 

分 类 号：R284.1[医药卫生—中药学]