慢性乙肝患者外周血HBcAg18-27V/I变异体特异性淋巴细胞分析  

Analysis of HBcAg18-27V/I Mutant Specific Cytotoxic T Lymphocytes (CTL)in Peripheral Blood of Patients with Chronic Hepatitis B

作　　者：马世武[1] 梁敏锋[1] 于乐成[1] 王战会[1] 周彬[1] 侯金林[1] 

机构地区：[1]南方医科大学南方医院感染内科,广州510515

出　　处：《热带医学杂志》2008年第2期104-107,共4页Journal of Tropical Medicine

基　　金：国家自然科学基金(No.30571662);国家基金一等博士后资助(No.2004036019)

摘　　要：目的分析不同HBcAg18-27V/I变异体特异性淋巴细胞的频数在慢性乙肝患者外周血中的差异。方法收集44例ALT≥2×ULN的慢性乙肝患者外周血淋巴细胞,通过PCR方法分析感染者病毒学背景(HBV基因型、HBcAg18-27V/I、HLA-A2基因型),应用流式细胞仪分别检测HBcAg18-27V-Tetramer(+)CD8(+)和HBcAg18-27I-Tetramer(+)CD8(+)的淋巴细胞频数。结果华南地区慢性乙肝患者主要为HLA-A2基因型(22/44);感染的HBV主要为B基因型(35/44)。其中HBcAg18-27为I变异体的占优势(43/44),在A2阳性组中,HBcAg18-27I-Tetramer(+)CD8(+)的淋巴细胞频数与HBcAg18-27V-Tetramer(+)CD8(+)的淋巴细胞频数(0.41±0.52VS0.28±0.43)比较差异有统计学意义(P<0.001)。结论在HBV基因型B/C为主的地区,应用HBcAg18-27V表位进行细胞免疫学研究而不考虑HBV病毒学背景的结果可能导致一定的偏差。Objective To analyze the differences between HBcAg18-27V-tetramer specific CTL and HBcAg18- 27I-tetramer specific CTL in peripheral blood of patients with chronic hepatitis B infection. Method Peripheral blood mononuclear cells （PBMC） were collected from 44 patients with chronic hepatitis B infection （ALT≥2×ULN）. The HBV and HLA genotyping, and the mutation of HBcAg18-27 were determined by polymerase chain reaction. CD8＋ HBcAg18-27V/I specific CTLs were analysed using flow cytometry. Result 22 out of 44 chronic hepatitis B patients had HLA-A2 genotype. The HBV genotype B was the major genotypes （33 out of 44） in these areas. In addition, HBcAg18-27I was the major variant （43/44）. For those patients with the HLA-A2 genotype, the frequency of HBcAg18-27I-tetramer specific CTL （0.41＋0.52） was significantly higher than the HBcAg18-27V specific CTL （0.28±0.43,P〈0.001）. Conclusion In the HBV （genotype B/C） prevalent area, serological analysis of HBcAg18- 27V is not sufficient. Additional virological analysis should also be performed.

关 键 词：肝炎 慢性 乙型 细胞毒性淋巴细胞 变异 表位 

分 类 号：R512.62[医药卫生—内科学]