大鼠肺缺血再灌注损伤不同时期基因表达谱的改变  

Cluster analysis of gene expression profile of pulmonary tissues in different phases of lung ischemi-a-reperfusion injury in rats

作　　者：李劲松[1] 聂君[1] 陈刚[1] 龚勇泉[1] 江科[1] 杨光海[1] 刘磊[1] 王建军[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院胸外科,武汉430030

出　　处：《中华实验外科杂志》2008年第2期200-202,共3页Chinese Journal of Experimental Surgery

基　　金：湖北省科技攻关计划项目（2007AA301B23-5）

摘　　要：目的探讨大鼠肺组织缺血再灌诱导的基因表达谱改变，为揭示肺缺血再灌注损伤的发生和保护机制提供新的线索和思路。方法采用无创血管夹夹闭左肺肺动脉与肺静脉，并于左肺膨胀时扎闭左主支气管构建大鼠肺缺血再灌反应动物模型。运用包含22226个大鼠基因点的Illumina RatRef-12全基因组表达谱微珠芯片检测大鼠肺缺血再灌处理后不同时间点（0、1、3、6、24h）的基因表达情况，并采用SOM算法将具有相似表达模式的基因进行聚类。结果缺血后再灌0、1、3、6、24h分别有648、340、711、1279、641个基因表达发生改变。具有相同表达模式的基因被聚为12类。结论肺缺血再灌可以诱导肺的基因表达谱发生改变，具有相似表达模式的基因可能具有相似的功能或相似的表达调控机制。Objective To provide new clues to induce some endogenous protective molecular mechanisms, the changes in gene expression profile induced by ischemia-reperfusion in pulmonary tissues of rats were investigated and the dynamic mechanism of pulmonary ischemia-reperfusion injury was elucidated. Methods Thirty male Wistar rats were randomly divided into 6 groups ： 5 ischemia-reperfusion （ I/ R） groups （ I/R 0-h,/./R 1-h, I/R 3-h,I/R 6-h, I/R 24-h） and control group （ n = 5 in each）. An in situ ischemia-reperfusion lung injury rat model was established by occluding hilus of lung. The RatRef-12 Expression Beadchip （22 226 gene probes per array） was used to analyze the pattern of gene expression in all groups. Results The results showed that 648,340,711,1279 and 641 genes were differentially expressed in I/R 0,1,3,6 and 24 h groups respectively. Clusters analysis identified 12 clusters of genes in which each cluster had similar expression pattern. Conclusion Many differentially expressed genes with different functions interacted each other to result in pulmonary ischemia-reperfusion injury. Genes with similar expression pattern could have the similar regulated mechanism.

关 键 词：肺 缺血 再灌注损伤 基因表达 基因芯片 

分 类 号：R285.5[医药卫生—中药学]