4’,5,7三羟基异黄酮抑制骨髓瘤基质细胞bcl-2,bcl-xl,CyclinD1,ICAM-1和IL-6基因表达  

Genistein inhibits the expression of bcl-2,bcl-xl,CyclinD1,ICAM-1 and IL-6 genes in bone marrow stromal cells of multiple myeloma

作　　者：何晖[1] 蔡冬梅[1] 翟明[1] 

机构地区：[1]中国医科大学第一临床学院血液科

出　　处：《中国新药杂志》2008年第3期215-218,共4页Chinese Journal of New Drugs

摘　　要：目的:研究4’,5,7三羟基异黄酮(Genistein)对人多发骨髓瘤(MM)基质细胞增殖和bcl-2,bcl-xl,CyclinD1,ICAM-1和IL-6基因表达的影响。方法:体外培养人多发骨髓瘤基质细胞,依据浓度梯度分别给予Genistein,用噻唑蓝染色法(MTT)观察Genistein对骨髓瘤基质细胞增殖的影响;10,20,40 mg.L-1Genistein与溶剂对照组分别作用骨髓瘤基质细胞48 h后,半定量RT-PCR法检测骨髓瘤基质细胞bcl-2,bcl-xl,CyclinD1,ICAM-1和IL-6基因表达。结果:Genistein作用骨髓瘤基质细胞48 h,随浓度增加,细胞增殖逐渐下降;10,20,40 mg.L-1Genistein处理组bcl-2,bcl-xl,CyclinD1,ICAM-1,IL-6基因mRNA的表达呈浓度依赖性下降,与溶剂对照组比较,差异有统计学意义(P<0.05,P<0.01)。结论:Genistein可能通过下调骨髓瘤基质细胞bcl-2,bcl-xl,CyclinD1,ICAM-1和IL-6基因mRNA的表达,抑制骨髓瘤基质细胞的生长。Objectives： To investigate the effects of genistein on proliferation and the expression of bcl-2, bcl-xl, CyclinD1, ICAM-1 and IL-6 genes in bone marrow stromal cells of human multiple myeloma. Methods： The growth of the bone marrow stromal cells was measured by MTT assay, and the gene expression was detected by RT-PCR. Results： After exposure to genistein for 48 h, proliferation of the stromal cells was inhibited in a concentration-dependent manner. The expressions of bcl-2, bcl-xl, CyclinD1, ICAM-1 and IL-6 genes were decreased in the stromal cells after treatment with genistein at 10, 20 and 40 mg·L^-1 for 48 h. Conclusion： Genistein can inhibit the proliferation by down-regulating the expression of bcl-2, bcl-xl, CyclinD1, ICAM-1 and IL-6 genes in bone marrow stromal cells of human multiple myeloma.

关 键 词：4 ’5 7三羟基异黄酮(Genistein) 骨髓瘤基质细胞 基因表达 

分 类 号：R512.6[医药卫生—内科学] R285.5[医药卫生—临床医学]