用液-液双相连续萃取装置测定β-CD包合物中当归挥发油含量的新方法  

Determination of angelica volatile oil in β-CD inclusion compound by liquid-liquid continuous biphase extraction

作　　者：杨会霞[1] 贺殿[1] 贾忠 杨建瑜[1] 鞠洋[1] 

机构地区：[1]兰州大学药学院 [2]兰州肺科医院,兰州730046

出　　处：《中国新药杂志》2008年第3期225-227,共3页Chinese Journal of New Drugs

基　　金：甘肃省新药临床前研究重点实验室开放基金项目(2004-07)

摘　　要：目的:考察液-液双相连续萃取法测定β-环糊精包合物中当归挥发油含量的可靠性。方法:采用液-液双相连续萃取自制装置,测定β-环糊精包合物中当归挥发油含量,将其测定结果与水蒸气蒸馏法的测定结果采用配对t检验进行统计学比较。结果:液-液双相连续萃取法测定β-环糊精包合物中当归挥发油含量为13.61%,RSD为2.09%;而水蒸气蒸馏法测得值为10.11%,RSD为4.97%,2种方法测得的当归挥发油含量比较,P<0.05,该差异有统计学意义。结论:液-液双相连续萃取法测定β-环糊精包合物中挥发油含量结果的准确度、重现性都较好。Objective： To determine the concentrations of angelica volatile oil in β-cyclodextrin（β-CD） inclusion compound by liquid-liquid continuous biphase extraction. Method：The concentrations oil in β-CD inclusion compound were determined by liquid-liquid continuous biphase extraction distillation method. The assay data was analyzed by paired-samples T Test. Result：The content of angelica volatile device with the wet of angelica volatile oil in β-CD inclusion compound determined by liquid-liquid continuous biphase extraction was 13.61% （RSD = 2.09% ）, and the content determined by wet distillation method was 10.11% （RSD = 4.97% ）. The difference was statistical significance （ P 〈 0.05 ）. Conclusion ： Both accuracy and reproducibility of the assay of angelica volatile oil in β-CD inclusion compound by liquid-liquid continuous biphase extraction were much better than the ones by wet distillation method.

关 键 词：液-液双相连续萃取 Β-环糊精包合物 当归 挥发油 

分 类 号：R284.2[医药卫生—中药学] R917.4[医药卫生—中医学]