蛋白质组学技术在药物肝毒性研究中的应用  

Application of the technologies of proteomics in drug-induced hepatotoxicity

作　　者：王莹[1,2] 王全军[1] 廖明阳[1] 

机构地区：[1]军事医学科学院毒物药物研究所,国家北京药物安全评价研究中心,北京100850 [2]辽宁医学院药物研究所,锦州121002

出　　处：《中国新药杂志》2008年第4期348-352,共5页Chinese Journal of New Drugs

摘　　要：蛋白质组学技术的发展促进了许多学科的发展,毒理蛋白质组学(toxicoproteomics)整合了经典毒理学、病理学和蛋白质差异表达分析技术,使毒理学研究上升到了一个新的高度。药物性肝损伤是肝脏疾病的一个重要原因,也是药物开发所面临的一项重大挑战。现就蛋白质组学的主要技术,包括双向凝胶电泳-质谱(2-DE-MS)、液相色谱-串联质谱(LC-MS-MS)、保留色谱-质谱(RC-MS)和蛋白质阵列,以及其在药物肝毒性研究中的应用,包括毒性蛋白标志物的筛选、毒性机制研究、毒性预测与毒性蛋白质数据库的建立方面进行综述。The improved technologies of coproteomics that integrates classic toxicology, pa proteomics thology and have promoted the developments in many fields. Toxiproteins express analysis technology has promoted toxicology to a new level. Drug-induced injury is one of the main etiological factors of liver diseases. Hepatotoxicity is a vital challenge during drug development. The main technologies of proteomics include two-dimensental gel electrophoresis mass spectrum （2-DE-MS）, liquid chromatography tandem mass spectrum （LC-MS-MS）, retentate chromatography-mass spectrometry （RC-MS） , protein arrays and so on. The application of technologies of proteomics in toxicity include screening toxicity biomarkers, exploring mechanism of toxic effect, predicting toxicity and establishing protein databases related to toxic effects. In the present article, we reviewed both the main technologies of proteomics and the application in drug hepatotoxicity research.

关 键 词：蛋白质组学 肝毒性 药物 

分 类 号：R965.3[医药卫生—药理学]