微米大黄炭对大鼠血小板功能和纤溶活性的影响  

Effects of micron rhubarb charcoal on the platelet function and fibrinolytic activity in rats

作　　者：时昭红[1] 张书[1] 郝建军[1] 王日权[2] 

机构地区：[1]武汉市第一医院消化内科,湖北省武汉市430022 [2]湖北中医学院中医系,湖北省武汉市430061

出　　处：《世界华人消化杂志》2008年第3期311-313,共3页World Chinese Journal of Digestology

摘　　要：目的:研究微米大黄炭对大鼠血小板功能和纤溶活性的影响.方法:以SD大鼠为研究对象,将其分为微米大黄炭大[0.8g/(100g·d)]、中[0.4g/(100g·d)]、小剂量组[0.2g/(100g·d)],阳性对照组云南白药[0.9g/(100g·d)]和空白对照组5组,给药6d,测定大鼠血小板聚集性,血栓素B_2(TXB_2)和6-酮前列腺素F_(1α)(6-keto-PGF_(1α))的含量,并测定大鼠组织型纤溶酶原激活物(t-PA)及其抑制剂(PAI-1)活性.结果:微米大黄炭能够明显提高血小板聚集性和TXB_2水平,而下调6-keto-PGF_(1α)水平,较空白对照组有统计学意义,而微米大黄炭大剂量组作用优于云南白药(64.19%±8.79% vs 56.82%±4.78%,273.35±24.86ng/L vs 238.35±47.30ng/L,15.21±2.71μg/L vs 13.12±3.09μg/L,P<0.05);微米大黄炭各剂量组对t-PA和PAI-1活性无明显影响.结论:微米大黄炭可能通过影响TXB_2和6-keto-PGF_(1α)的含量来实现对血小板活化作用的调节.AIM： To study the effects of micron rhubarb charcoal on platelet function and fibrinolytic activity in rats. METHODS： SD rats were divided into control group, Yunan Baiyao group[0.9 g/（100 g·d）] and high [0.8 g/（100 g·d）], normal [0.4 g/（100 g·d）]and low [0.2 g/（100 g·d）] micron rhubarb charcoal dosage groups（ig for six days）. The effects of micron rhubarb charcoal on platelet aggregation, TXB2, 6-keto-PGF1α and the activity of tissue fibrinolytic activating factor （t-PA） and its inhibitors （PAI-1） in SD rats were studied. RESULTS： Compared with normal rats treated with Yunan Baiyao, the platelet aggregation and TXB2 level were increased while the 6-keto- PGF1α level was decreased in rats treated with micron rhubarb charcoal （P〈0.05）, suggesting that high micron rhubarb charcoal dosage had a better effect than Yunan Baiyao on platelet function and fibrinolytic activity in rats （64.19% ± 8.79% vs 56.82% ± 4.78%, 273.35 ± 24.86 ng/L vs 238.35 ± 47.30 ng/L, 15.21 ± 2.71 μg/L vs 13.12 ± 3.09 μg/L, P〈0.05）. However, micron rhubarb charcoal had no effect on the activity of t-PA and its inhibitor PAL. CONCLUSION： Micron rhubarb charcoal regulates platelet aggregation by decreasing the level of TXB2 and 6-Keto-PGF1α and its effects on hemostasia are better than Yunan Baiyao.

关 键 词：微米大黄炭 血小板聚集率 血栓烷素B2 6酮前列腺素F1α 纤溶活性 酶联免疫吸附双抗体夹心法 

分 类 号：R285[医药卫生—中药学]