体外培养大鼠软骨细胞老化的机制初探  

Preliminary probe into aging mechanism of chondrocyte in the rats in vitro

作　　者：陈晓东[1] 林建华[2] 

机构地区：[1]深圳平乐骨伤科医院,广东深圳518010 [2]福建医科大学附属第一医院骨科

出　　处：《中国老年学杂志》2008年第4期324-327,共4页Chinese Journal of Gerontology

基　　金：福建省科技计划重大资助项目(2004Y018)

摘　　要：目的对体外传代培养的大鼠软骨细胞进行相关因子检测,初步探讨其复制性老化(传代培养出现的老化表现)的机制。方法对连续培养的第二、三、四代软骨细胞进行实验,采用免疫细胞化学检测p16、pRb、E2F、CyclinD、CDK4等因子表达水平,TRAP-ELISA检测端粒酶活性.观察软骨细胞老化过程中各因子的变化。结果随着细胞复制性老化p16、pRb、CyclinD表达水平显著上升(P<0.01),E2F、CDK4、端粒酶表达水平显著下降(P<0.01)。结论体外培养软骨细胞老化过程与p16、pRb、CyclinD表达增加,E2F、CDK4、端粒酶表达下降密切相关。Objective To determine the correlated factors of chondrocyte in the rats in vitro to explore its replicative senescence mechanism. Methods The expression levels of p16, pRb, E2F, CyelinD and CDK4 were determined by immunoeytoehemistry and telomerase activity were detected by TRAP-ELISA in the second, third and fourth generation ehondroeytes of consecutive cultivation to observe their changes in senescence process. Results The expression levels of p16, pRb, CyclinD significantly increased （ P 〈 0. 01 ） , those of E2F, CDK4 and telomerase obviously decreased （ P 〈 0. 01 ） with replicative senescence in chondrocytes. Conclusions The senescence process of ehondrocyte in the rats in vitro is closely related to the increased expressions of p16, pRb and CyelinD and the decreased expressions of E2F, CDK4 and telomerase.

关 键 词：软骨细胞 老化 机制 

分 类 号：R339.03[医药卫生—人体生理学] Q255[医药卫生—基础医学]