骨髓间质细胞中组织定向干细胞的鉴定  

Identification of the tissue committed stem cells among bone marrow stromal cells

作　　者：张林[1] 李玉泉[1] 张炎[1] 杨向群[1] 张传森[1] 

机构地区：[1]第二军医大学解剖学教研室,生物医学工程研究所,上海200433

出　　处：《解剖学杂志》2008年第1期8-10,共3页Chinese Journal of Anatomy

基　　金：国家自然科学基金(30200053);军队"十一五"规划项目(06MA171)

摘　　要：目的:验证骨髓中存在组织定向干细胞(TCSCs),为心血管组织工程提供理想的种子细胞来源。方法:采用SDF-1α梯度趋化从骨髓中分离CXCR4阳性细胞,免疫细胞化学法检测CXCR4与PDGFR-β表达,流式细胞仪检测PDGFR-β阳性细胞,从而确定平滑肌祖细胞(SMPCs)的比例;从骨髓分离培养内皮祖细胞(EPCs),免疫细胞化学和流式细胞仪检测FLK-1、CD34、CXCR4与vWF的表达情况。结果:SDF-1α趋化分离出的细胞几乎都为CXCR4阳性,其中4·67%的细胞呈PDGFR-β阳性;EPCs形态圆形或近三角形贴壁生长,原代细胞CXCR4、VEGFR-2、CD34明显阳性,传代细胞vWF阳性。结论:在骨髓基质细胞中存在SMPCs和EPCs等TCSCs。Objective： To validate the existence of the tissue committed stem cells （TCSCs） among the bone marrow stroreal cells （BMSCs） and provide better sources of seeding cells for cardiovascular tissue engineering. Methods： To identify the smooth muscle progenitor cells, CXCR4 positive cells were isolated by SDF-1α chemotaxis assay from the bone marrow of rats and expressions of CXCR4 and PDGFR-β of cells were examined by immunocytochemistry and flow cytometry, respectively. Expressions of CXCR4, VEGFR-2, CD34 and vWF were examined to identify the endothelial progenitor cells （EPCs）. Results：The cells isolated by chemotaxis assay of SDF-1α gradient were CXCR4 positive and there were 4.67% PDGFR-β positive cells among them. EPCs were round or triangle and they could reach confluent on 7 d. CXCR4, VEGFR- 2 and CD34 were positive in primary cells while VEGFR-2 and CD34 became weak and vWF was positive in passaged cells. Conclusion： There were TCSCs such as SMPCs and EPCs among BMSCs.

关 键 词：组织定向干细胞 平滑肌祖细胞 内皮祖细胞 骨髓基质细胞 大鼠 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]