合成紫花茄皂苷对体外培养肝癌细胞的增殖抑制作用  

Anti-proliferation effect of synthetic indioside from Solanum indicum L. on human hepatocarcinoma cells

作　　者：马朋[1] 曹同涛[1] 宋晓冬[1] 张页[2] 

机构地区：[1]滨州医学院生物学教研室,滨州256603 [2]北京大学医学部细胞生物学系,北京100083

出　　处：《解剖学杂志》2008年第1期25-27,共3页Chinese Journal of Anatomy

基　　金：国家自然科学基金(30330690);滨州医学院科技计划(BY2005KJ09)

摘　　要：目的:探讨合成紫花茄皂苷对人肝癌细胞株BEL-7402的增殖抑制及凋亡诱导作用。方法:采用酸性磷酸酶(ACP)法检测细胞增殖抑制率;电镜观测BEL-7402细胞形态变化;流式细胞仪检测细胞凋亡率。结果:合成紫花茄皂苷对人肝癌BEL-7402细胞有明显的增殖抑制作用,其72h的IC50为6·5μg/ml。皂苷可引起细胞形态的明显改变。细胞经皂苷作用6h和12h后,凋亡率显著增加。结论:合成紫花茄皂苷对人肝癌细胞的增殖抑制呈浓度相关性,可诱导肿瘤细胞凋亡。Objective： To investigate the anti-proliferation effect and apoptosis-inducing effect of synthetic indioside from Solanum indicum L. on human hepatocarcinoma BEL-7402 cells. Methods： Acid phosphatase （ACP） assay, electron microscopy and flow cytometry were used. Results： The solanum saponin significantly inhibited proliferation of BEL-7402 cells with IC50 value of 6.5 μg·ml^-1 for 72 hours. Typical morphological change were observed by electron microscopy on the indioside treated cells. Apparent enhancement in apoptosis rate was observed using flow cytometry, after BEL-7402 cells had been exposed to the indioside for 6 hours and 12 hours, Conclusion： The indioside has dose-dependent inhibition effects on proliferation of human hepatocarcinoma cells, and can induce apoptosis

关 键 词：紫花茄 皂苷 细胞增殖 细胞凋亡 肝癌细胞 

分 类 号：R285[医药卫生—中药学] R735.7[医药卫生—中医学]