大鼠股直肌肌纤维型组成及分布研究  

Study on the composition and distribution of fiber types in rat rectus femoris muscle

作　　者：张潜[1] 章涛[2] 王正义[3] 薛黔[1] 

机构地区：[1]遵义医学院解剖学教研室,贵州遵义563003 [2]遵义医学院贵州省细胞工程重点实验室,贵州遵义563003 [3]重庆医科大学基础医学研究所,重庆400016

出　　处：《遵义医学院学报》2007年第4期456-457,460,共3页Journal of Zunyi Medical University

基　　金：贵州省科学技术基金资助项目(黔科合J字[2005]2058号)

摘　　要：目的探讨大鼠股直肌的肌纤维型组成和分布,以了解该肌功能。方法取成年SD大鼠股直肌组织进行冰冻切片(8μm厚),采用Guth-Samaha肌球蛋白ATP酶组织化学染色法并稍做改良,对其进行肌纤维分型研究。结果大鼠股直肌经肌球蛋白ATP酶组织化学染色后可明确分出2种肌纤维型,即白色的Ⅰ型纤维(慢缩纤维)和深褐色的Ⅱ型纤维(快缩纤维),2种肌纤维在肌内呈棋盘样均匀分布;图像分析仪计数Ⅰ纤维占(24.6±3.1)%,Ⅱ型纤维比例为(75.4±6.3)%,2者比较差异显著(P<0.01)。结论大鼠股直肌的肌纤维组成以Ⅱ型纤维为主,属于力量速度型肌。Objective To study the fiber types composition and distribution of rat rectus femoris muscle and evaluate the muscle＇s function. Methods Rectus femoris muscle from both sides of 10 adult SD rats were studies . 8 μm thickness of the muscles＇ frozen transverse sections were stained for myosin ATPase by Guth ＆ Samaha reported procedure after being reformed partially. Results The rectus femoris muscle derived from SD rats can be clearly identified as type Ⅰ （slow-twitch fibers）and type Ⅱ （fast twitch fibers） fiber types by myosin ATPase staining and the former type looks brightly while the latter darkly. The distribution of this two types of muscle fibers in the muscle were uniform and chessboard like. The percentage composition of type Ⅱ fibers was （75.4±6.3）% ,much higher than that of type Ⅰ fibers （24.6±3.1）% （P〈0.01）. Conclusions Rat rectus femoris muscle is dominated by type Ⅱ fibers which imply that the muscle participate more in fast stepping movement.

关 键 词：股直机 肌纤维型 肌球蛋白ATP酶 大鼠 

分 类 号：Q445[生物学—生理学] Q954