砷染毒大鼠生精细胞Bcl-2表达的变化  

The Effects of As_2O_3 to Bcl-2 expression of rat spermatogenic cells

作　　者：舒小林[1] 陈伟[1] 陆祥[1] 李季蓉[1] 

机构地区：[1]遵义医学院组织与胚胎学教研室,贵州遵义563003

出　　处：《遵义医学院学报》2007年第4期461-463,466,共4页Journal of Zunyi Medical University

基　　金：贵州省国际科技合作重点项目资助

摘　　要：目的观察不同剂量的三氧化二砷(As2O3)对成年大鼠生精细胞Bcl-2表达的影响。方法40只健康雄性SD大鼠随机分成4组,分别为0.375 mg/kg、0.75 mg/kg、1.5 mg/kg 3个染毒组和对照组,灌胃法连续给药16w后对各组大鼠计算睾丸脏器系数、精子头计数,并计算每日精子生成量(DSP)。采用免疫组化法检测大鼠生精细胞Bcl-2表达的情况。结果①中剂量组(0.75 mg/kg)和高剂量组(1.5 mg/kg)睾丸精子头计数和DSP均低于对照组(P<0.05);②与对照组相比,中、高剂量组大鼠生精细胞Bcl-2阳性产物表达明显降低(P<0.01);③生精细胞Bcl-2阳性率与每日精子生成量呈正相关(r=0.589,P<0.01)。结论一定剂量的As2O3可通过抑制生精细胞Bcl-2的表达而导致精子生成量的减少,产生雄性生殖毒性。Objective To observe the expression of Bcl-2 in spermatogenic cells after aniso-doses arsenic （As2O3） administration in the testes of the adult male rats. Methods 40 healthy male Sprague-Dawle rats were divided randomly into four groups and they were administered respectively with 0 （control group）,0.375、0.75 、1.5 mg .kg^-1 body weight of As2O3 by intragnstrie administration consecutively for 16 weeks. The numbers of testieular sperm head were counted and the coefficient of testieular viscera , daily sperm production （DSP） were calculated in every group. The Bcl-2 expression in spermatogenie cells of different grades were located and quantitated by method of immunohistochemistry. Results （1）In the testes of As2O3-treated rats, The coefficient of testieular viscera , the number of testieular sperm head and daily sperm production in middle and high doses group decreased significantly than that of control（P〈0.05）;（2）The expression of Bcl-2 in middle dose group and high dose group decreased significantly compared with that in the control group （P〈0.01）;（3）The dependability analysis between DSP and the expression of Bcl-2 in spermatogenie cells showed a positive correlation （r= 0.589,P〈0.01）. Conclusions One mechanisms of male reproduction toxicity of As2O3 might be that the expression of Bcl-2 is inhibited ,which induces decrese of quantity of sperm cells.

关 键 词：AS2O3 BCL-2 生精细胞 大鼠 

分 类 号：R114[医药卫生—卫生毒理学]