贵州小型猪复合因素所致肝纤维化模型的建立  

Establish Meat of Hepatic Fibrosis Model of Guizhou Minipig by Multiplex Factor

作　　者：赵菊花[1] 吴曙光[2] 巫全胜[2] 郭科男[2] 董小君[2] 魏剑林[3] 钱宁[2] 

机构地区：[1]四川中医药高等专科学校,绵阳621000 [2]贵阳中医学院,550002 [3]四川省剑阁县人民医院,628300

出　　处：《实验动物科学》2007年第6期74-77,68,共5页Laboratory Animal Science

基　　金：贵州省科技厅资助项目(编号:2004JN036)

摘　　要：建立贵州小型猪复合因素所致的肝纤维化模型,为肝纤维化机制和治疗研究提供基础。方法贵州小型猪16头,雌雄各半,随机分为对照组和实验组。对照组每周两次给予溶剂橄榄油和生理盐水,实验组每周两次分别腹腔注射给予40%的四氯化碳(CCl4)橄榄油和兔血清各0.5 mL/kg,共9周,建立复合因素所致的肝纤维化模型。实验结束时,取血清查肝功ALT、AST、TP、ALB、A/G,取肝组织邻片做常规HE染色、Masson染色和Van Gieson染色,光镜和电镜下观察病理变化。结果染毒9周后,实验组血清ALT、AST比对照组明显增高(t值分别为0.0001、0.0004,P<0.01),ALB、A/G比对照组明显降低(t值分别为0.001、0.003,P<0.01);实验组肝组织光镜和电镜下观察病理切片均较对照组有明显的改变。结论复合因素CCl4、兔血清腹腔注射可导致贵州小型猪肝纤维化。Objective To establish the hepatic fibrotic model of Guizhou minipig by multiplex factor. Methods Sixteen Guizhou minipig （8 females and 8 males） were divided into two groups including one control group and one experiment group at random. Control group was injected into peritoneum with olive oil and 0.9% NS,0.5 mL/kg twice a week, fibrosis model of experiment group was duplicated by peritoneal injection of 40 % carbon tetrachloride and rabbit serum 0.5 mL/kg twice a week,for nine weeks. They were sacrificed at the end of the ninth weeks,then serum marker levels of liver function of ALT, AST, TP, ALB, A/G were determined. The liver were taken out and paraffin sections were stained by hematoxylin-eosin, Masson, Van Gieson, and were examined under light microscope （LM）. The liver from experimental group were observed under electron microscopy（EM）. Results Compared with the control group, serum ALT,AST activity of experiment group elevated significantly（ P 〈 0.01 ）, ALB, A/G content decreased markedly（ P 〈 0.01） ;the pathological changes of liver of experiment group were observed under LM and EM. Conclusion Hepatic fibrosis model of Guizhou mini-pig was duplicated by peritoneal injection of 40% carbon tetrachloride and rabbit serum.

关 键 词：贵州小型猪 复合因素 肝纤维化 模型 

分 类 号：Q95-33[生物学—动物学]