二硫化碳对大鼠周围神经轴浆运输和能量代谢的毒作用效应  

Toxic Effects of Carbon Disulfide on Axonal Transport and Energy Metabolism in Peripheral Nerves in Rats

作　　者：鲁洁波[1,2] 黄金祥[1,2] 丁茂柏[1,2] 张寿林 宋文佳[1,2] 姜国华 

机构地区：[1]中国预防医学科学院劳动卫生与职业病研究所 [2]北京师范大学分析测试中心

出　　处：《中国工业医学杂志》1997年第4期198-201,共4页Chinese Journal of Industrial Medicine

摘　　要：为研究二硫化碳（ＣＳ２）对周围神经系统的毒作用效应，用亚慢性染毒方式建立ＣＳ２所致周围神经病的大鼠模型后，采用同位素示踪技术和反向高效液相色谱法（ＲＰ－ＨＰＬＣ），分别测定了染毒组和对照组大鼠的坐骨神经中轴浆运输和能量代谢的变化。结果显示，同位素标记物３Ｈ－亮氨酸在亚慢性ＣＳ２中毒大鼠坐骨神经的运动和感觉神经中的运行距离均比对照组短；坐骨神经中ＡＴＰ、ＡＤＰ和ＡＭＰ的含量也均低于对照组。本研究表明ＣＳ２引起大鼠的周围神经损伤出现轴浆运输和能量代谢功能障碍。这些改变与ＣＳ２中毒性周围神经病在时间－剂量－反应上的关系尚待深入研究。A model of peripheral neuropathy in rats caused by subchronic exposure to carbon disulfide(CS 2)was established to study the toxic effects of CS 2 on peripheral nervous system,and changes in axonal transport and energy metabolism in sciatic nerve in the exposed and control rats were measured by isotope tracing technique and reversed phase high performance liquid chromatography.Results showed that the transport distance of isotope labelling 3H leucine in motor and sensory nerves of the rats with subchronic CS 2 poisoning reduced significantly,as compared with that of the controls.Contents of adenosine triphosphate(ATP),adenosine diphosphate(ADP)and adenosine monophosphate(AMP)in sciatic nerve of the exposed rats were lower than those of the controls.the research indicated that peripheral nerve damage caused by CS 2 could lead functional disturbance of axonal transport and energy metabolism.Dose response relationship between these changes and toxic neuropathy caused by CS 2 should be studied further.

关 键 词：二硫化碳 轴浆运输 能量代谢 周围神经病 

分 类 号：R135.1[医药卫生—劳动卫生] R745[医药卫生—公共卫生与预防医学]