ATP-MgCl_2心停搏液对发育未成熟兔心肌缺血再灌注后能量代谢的影响  

Effect of ATPMgCl 2 cardioplegia solution on energy metabolism of myocardium during ischemiareperfusion in immature rabbit's heart

作　　者：陈林[1] 刘欲团[1] 肖颖彬[1] 董建菊 

机构地区：[1]第三军医大学新桥医院

出　　处：《中国危重病急救医学》1997年第8期454-455,共2页Chinese Critical Care Medicine

摘　　要：目的：探讨ＡＴＰＭｇＣｌ２心停搏液对发育未成熟心肌的保护作用。方法：采用离体心作功模型，观察局部单低温（１０～１５℃）、改良Ｔｈｏｍａｓ液和ＡＴＰＭｇＣｌ２心停搏液加低温单剂灌注对未成熟兔心肌缺血１２０分钟、再灌注６０分钟后能量代谢变化的影响。结果：缺血再灌注后，ＡＴＰＭｇＣｌ２心停搏液灌注组游离腺苷酸总量（ＡＮＰ）、三磷酸腺苷（ＡＴＰ）、二磷酸腺苷（ＡＤＰ）含量均优于其它２组；而一磷酸腺苷（ＡＭＰ）、心肌线粒体丙二醛（ＭＤＡ）及冠状动脉流出液中肌酸磷酸激酶（ＣＰＫ）、乳酸脱氢酶（ＬＤＨ）和谷草转氨酶（ＧＯＴ）含量均低于另２组。结论：ＡＴＰＭｇＣｌ２心停搏液具有改善未成熟兔心肌缺血再灌注后能量代谢的作用。Objective:To investigate the protective effect of ATPMgCl 2 cardioplegia solution on immature myocardium.Methods:We used an isolated working heart model from 3￣4 weekoldrabbit's heart to examine protective effects of various treatment measures,including topical cooling alone (10￣15℃,group Ⅰ),singledose modified Thomas solution plus cooling (group Ⅱ),and singledose ATPMgCl 2 cardioplegia solution plus cooling (group Ⅲ).Results:Following 120 minutes of global ischemia and followed by 60 minutes of reperfusion,it showed that there were marked high nucleotide (such as ATP,ADP and ANP) contents of myocardium and a low AMP content in myocardium in group Ⅲ compared to groupⅠ and Ⅱ.Also,coronary leakage of lactic dehydrogenase(LDH),glutamic oxaloacetic transaminase(GOT),creatine phosphokinase(CPK),and malondialdehyde (MDA) of myocardial mitochondria were much lower in group Ⅲ than those in other two groups.Conclusions:Our data suggest that ATPMgCl 2 cardioplegia solution may improve the energy metabolism in immature rabbit's heart after ischemiareperfusion.

关 键 词：缺血-再灌注 先天性心脏病 ATP-MGCL2 心停搏液 

分 类 号：R541.105[医药卫生—心血管疾病] R654.2[医药卫生—内科学]