HPLC法测定银杏叶中6种黄酮成分的含量  

HPLC DETERMINATION OF SIX FLAVONOID CONSTITUENTS IN GINKGO BILOBA LEAVES

作　　者：池静端[1] 何秀峰[1] 刘爱茹[1] 

机构地区：[1]中国医学科学院中国协和医科大学药物研究所

出　　处：《药学学报》1997年第8期625-628,共4页Acta Pharmaceutica Sinica

摘　　要：用反相高效液相色谱法测定了银杏叶中６种黄酮成分即槲皮素（ｑｕｅｒｃｅｔｉｎ）、异鼠李素（ｉｓｏｒｈａｍｎｅｔｉｎ）、山萘酚（ｋａｅｍｐｆｅｒｏｌ）、白果黄素（ｂｉｌｏｂｅｔｉｎ）、银杏黄素（ｇｉｎｋｇｅｔｉｎ）、西阿多黄素（ｓｃｉａｄｏｐｉｔｙｓｉｎ）。单酚酸Ｂ（ｓａｌｖｉａｎｏｌｉｃａｃｉｄＢ）为内标。并对１８个样品进行了测定。色谱柱为ＺｏｒｂａｘＯＤＳ柱，流动相：Ａ．ＭｅＯＨ，Ｂ．ＴＨＦ—Ｈ２Ｏ—ＨＣＯＯＨ（３４∶６５∶１），梯度洗脱。流速：１０ｍｌ·ｍｉｎ－１，检测波长：３５０ｎｍ。线性范围０１０～２５７μｇ，相关系数γ＝０９９９４～０９９９８，回收率９８０％～１０２０％。本法准确、快速、重现性好。Six flavonoid constituents (quercetin, isorhamnetin, kaempferol, bilobetin, ginkgetin and sciadopitysin) were isolated from Ginkgo biloba leaves and determined by reversed phase HPLC using salvianolic acid B as internal standard. The column employed was Zorbax ODS (150 mm×4 mm ID, 5 μm).The mobile phase consisted of solvent A (methanol) and solvent B [tetrahydrofuran — water — formic acid (34∶65∶1)] for gradient elution. The flow rate was 1 ml·min -1 and detection was effected at 350 nm. This method is accurate, rapid and reproducible. Analytical data for various samples were given.

关 键 词：银杏 黄酮 HPLC 

分 类 号：R284.1[医药卫生—中药学]