Smad2基因敲除小鼠冷冻胚胎库的建立  

Establishment of Embryo Bank for Smad2 Gene Knock-out Mice

作　　者：杨秀兰[1] 李慕[1] 江其辉[1] 尚士臣[1] 时彦胜[1] 孙岩松[1] 苏玉虹[2] 李文龙[1] 

机构地区：[1]军事医学科学院实验动物中心,北京100071 [2]锦州医学院辽宁省高校分子细胞生物学与新药开发重点实验室,锦州121000

出　　处：《实验动物科学》2008年第1期1-5,共5页Laboratory Animal Science

基　　金：北京市科研条件专项资助(编号:Z0004100040691-6);辽宁省科技厅课题(编号:2005408002)

摘　　要：目的建立Smad2基因敲除小鼠胚胎库。方法利用OPS法对Smad2基因敲除小鼠胚胎进行玻璃化冷冻保存,并比较不同杂交组合小鼠的超数排卵数、解冻胚胎的复苏率及发育率。结果两组不同杂交组合(Smad2+/-♂×Smad2+/-♀和Smad2+/-♂×Smad2+/+♀)小鼠平均超排卵数分别为14.13枚和24.60枚;复苏率分别为90.16%和91.67%;囊胚发育率分别为73.08%和77.05%。这些结果表明Smad2基因敲除杂合子母鼠的超排数量明显低于野生型母鼠的超排数量,而二者胚胎解冻后的复苏率和囊胚发育率没有显著差异。因此我们主要通过对野生型小鼠超数排卵,然后与Smad2基因敲除杂合子雄鼠交配的方法获取胚胎,进行玻璃化冷冻保存,现已冻存胚胎1256枚。结论成功建立了Smad2基因敲除小鼠胚胎库。Objective To establish embryo bank for Smad2 gene knock-out mice. Methods The OPS vitrification was used to cryopreservation of morula and blastocyst embryos of the Smad2 gene knock-out mice . We compare the results of superovulatory treatment, recovery rate and survival rate in different groups. Results The average numbers of embryo production of Smad2^＋/- and Smad^2＋/＋ were 14.13 and 24.60 respectively after superovulatory treatment. The recovery rates of the two group embryos were 90.16% and 91.67% respectively. The rates of development were 73.08% and 77.05 % in vitro respectively after thawing. The results indicated that the numbers of superovulatory of Smad2^ ＋/- were lower than the ones of Smad2^＋/＋ , the rates of：recovery and the rate of survival were no different between the two group mouse after thawing. We have cryopreserved 1256 embryos of the Smad2 gene knock-out mice by OPS vitrification. Conclusion We establish the embryo bank for the Smad2 gene knock-out mice.

关 键 词：SMAD2 基因敲除小鼠 胚胎 玻璃化冷冻 

分 类 号：Q954.4[生物学—动物学]