结核分枝杆菌38 kD重组蛋白的纯化及血清学诊断应用价值的研究  

Purification of recombinant 38 kD protein and application in serodiagnosis of tuberculosis

作　　者：阳幼荣[1] 吴雪琼[1] 张俊仙[1] 梁艳[1] 李洪敏[1] 王兰[1] 张翠英[1] 孟祥红[1] 朱琰[1] 

机构地区：[1]解放军第309医院结核病中心研究室,北京100091

出　　处：《中国现代医学杂志》2008年第4期426-427,431,共3页China Journal of Modern Medicine

基　　金：国家自然科学基金资助项目(30471648)

摘　　要：目的纯化获得结核分枝杆菌重组38kD蛋白,并评价其在血清学诊断方面的应用价值。方法应用亲和层析方法纯化结核分枝杆菌重组38kD蛋白,应用ELISA方法检测220例血清中的抗结核抗体。结果结核分枝杆菌重组38kD蛋白以包涵体形式表达,以48名正常人血清OD492+2S为正常限值,57例PPD强阳性血清,47例菌阳结核病人血清和68例菌阴结核病人血清阳性检出率分别为13.8%、97.87%和83.82%。结论结核分枝杆菌38kD重组蛋白以包涵体形式高效表达,具有较强的抗原特异性和免疫反应性。[Objective] To obtain the recombinant 38 kD protein, and evaluate its value in serodiagnosis of tuberculosis. [Methods] The recombinant 38 kD protein was purified by metal chelation chromatography, and its immunogenicity was analyzed in 220 sera by ELISA. [Result] The recombinant 38 kD protein existed in inclusion bodies of TB bacilli. The positive cutoff value was determined as OD492 ＋2S of 48 sera from PPD-negative healthy people detected by ELISA. Of 57 sera from PPD-positive people, 47 sera from smear-positive tuberculosis patients and 68 sera from smear-negative tuberculosis patients, the positive rates of anti-tuberculosis antibodies were 13.8%, 97.87% and 83.82%, respectively. [Conclusion] The recombinant 38 kD protein could be highly expressed in the form of inclusion bodies in TB bacilli. It had good antigenic specificity and immunoreactivity.

关 键 词：结核分枝杆菌 抗原 38 kD重组蛋白 血清学诊断 

分 类 号：R446.112.9[医药卫生—诊断学]