核因子KB、肿瘤坏死因子在糖尿病大鼠肺组织中活性的变化  

Changes of activity of TNF-a and NF-kB in lungs of diabetic rats

作　　者：陈瑛[1] 张康兰[2] 丁吉元[3] 

机构地区：[1]济南军区青岛第一疗养院特检科,山东青岛266071 [2]济南军区总医院眼科,山东济南250031 [3]济南军区总医院病理科,山东济南250031

出　　处：《中国现代医学杂志》2008年第4期440-442,共3页China Journal of Modern Medicine

摘　　要：目的连续6个月,观测糖尿病(DM)大鼠肺组织的病理改变及核因子KB p65(NF-kBp65)、肿瘤坏死因子-a(TNF-a)活性的变化。方法采用右下腹腔内一次性注射Streptozotocin 60mg/kg诱导Wistar大鼠模型26只,分别于成模后1、3及6个月观察肺组织的病理改变,采用免疫组织化学检测NF-kBp65、TNF-a在DM大鼠肺组织表达变化。结果DM大鼠3个月毛细血管及肺泡间隔增厚,肺间质胶原成分增多,6个月肺泡间隔明显增厚,细胞数目增加,出现肺实变;NF-kBp65、TNF-a在肺组织的各部分中表达均随病程的的延长而显著增强。结论DM大鼠肺组织高糖环境下,肺组织发生不同程度的病理改变,NF-kBp65、TNF-a激活表达,提示DM肺部并发症的发生和发展可能与NF-kBp65、TNF-a表达有关。[Objective] To observe the changes of pathology and the activity of tumour necrosis factor -α （ TNF- α ） and nuclear factor-kB（NF-kB） in diabetic rat lungs in 6 months. [Methods] A total of 26 streptozotocins- in- duced diabetic rats were included, HE, immunohistochemistry of TNF- α and NF-kB were taken in the 1, 3, 6 months respectively. [Results] Thicked pulmonary capillary basal lamina and increased number of fibre were observed after 3 month. The expression of TNF- α and NF-kB was found in diabetic rats and increased with the devel- opment of diabetes. [Conclusion] The expression of TNF-α and NF-kB may be related to complication of lung in the diabetic rats.

关 键 词：糖尿病 NF—kBp65 TNF—α 肺 

分 类 号：R587.2[医药卫生—内分泌]