雌激素对颅脑创伤大鼠皮质神经细胞c-fos表达及凋亡的影响  

Investigation on activation of estradiol benzoate on nerve cell c-fos and apoptosis of rats cortex following traumatic brain injury

作　　者：高俊玲[1] 陈万欣[1] 田艳霞[1] 路文婷[1] 张志民[2] 张宇新[1] 崔建忠 

机构地区：[1]华北煤炭医学院组胚教研室,河北唐山063000 [2]承德医学院附属医院肾脏内科,河北承德067000 [3]河北唐山市工人医院神经外科,河北唐山063000

出　　处：《中国现代医学杂志》2008年第4期458-461,465,共5页China Journal of Modern Medicine

基　　金：河北省科技领军人才基金(博士基金)(No:06547008D-7);中国人事部留学人员优秀科技项目(No:2007-17)

摘　　要：目的探讨雌激素在颅脑创伤中的作用。方法按照Marmarou方法建立大鼠创伤性脑损伤(TBI)模型,采用硫代巴比妥酸法测定氧自由基中间产物丙二醛(MDA),免疫组化方法检测细胞凋亡相关基因c-fos的表达,原位末端标记(TUNEL)法检测凋亡细胞。结果雌激素治疗组皮质MDA和c-fos的表达及TUNEL阳性细胞均低于模型组。结论大鼠脑创伤后雌激素通过抗氧化作用抑制神经细胞凋亡而起到脑保护作用。[Objective] To explore the effect of estradiol benzoate on traumatic brain injury （TBI）. [Methods] According to Mamarou established the TBI model and measured MDA by thio-barbituric acid, c-fos by immunohistochemical and apoptosis by TUNEL. [Results] The value of MDA, c-fos and TUNEL posit/re cells of estradiol benzoate group were lower than TBI group. [Conclusion] Estradiol benzoate attenuate the apoptosis of the nerve cells by reducing oxygen free radical following traumatic brain injury.

关 键 词：大鼠 苯甲酸雌二醇 氧自由基 颅脑创伤 c—fos 凋亡 

分 类 号：R651.15[医药卫生—外科学]