寡核苷酸芯片技术在甲型流感及登革病毒检测分型中的初步应用  

Application of oligonucleotide microarray in detecting and subtyping of influenza A virus and dengue virus

作　　者：张海燕[1] 马文丽[1] 黄吉城[2] 商涛[1] 郑文岭 

机构地区：[1]南方医科大学基因工程研究所 [2]广东出入境检验检疫局检验检疫技术中心 [3]华南基因组中心

出　　处：《山东医药》2008年第4期22-23,共2页Shandong Medical Journal

基　　金：国家质量监督检验检疫总局科技计划资助项目(2006IK168)

摘　　要：目的建立寡核苷酸芯片检测技术,对甲型流感病毒及登革病毒进行早期检测及分型。方法根据Genebank中病毒基因组序列的保守区域,设计病毒的特异性检测探针,制备病毒检测芯片;利用限制性显示技术标记样品中的病毒靶序列,将标记产物与芯片杂交、清洗、扫描及结果分析。结果该方法可以正确检测到细胞培养所获得的甲型(H1N1,H3N2)流感病毒和登革四型病毒。结论寡核苷酸芯片技术可应用于多种病毒及其亚型的早期检测。Objective To establish an oligonueleofide mieroarray method for early detecting and subtyping of influenza A virus and dengue virus. Methods Specific detection probes for each virus and virus detection array were designed by the conserved sequence of virus genome sequence in genebank. Virus target sequences were marked by restriction display technology. Hybridization products of marking product and array were cleaned, scanned and analyzed. Results Influenza A virus （H1N1 ,H3N2） and dengue virus in the cultured cell could be detected correctly by oligonucleotide microarray. Conclusion Oligonueleotide microarray technology can be used to detect several viruses and their subtypes.

关 键 词：流感病毒属A型 登革热病毒 基因芯片 限制性显示技术 

分 类 号：R511.7[医药卫生—内科学] R373.3[医药卫生—临床医学]