猪日本脑炎DNA疫苗的构建与鉴定  

Construction and identification of DNA vaccine against Japanese encephalitis in swine

作　　者：唐青海[1] 董志强[2] 乔宪凤 郑新民 华文君 何维明[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]中国科学院广州生物医药与健康研究院,广东广州510620 [3]湖北省畜牧兽医研究所,武汉430064

出　　处：《中国动物检疫》2008年第3期26-28,共3页China Animal Health Inspection

基　　金：湖北省自然科学基金项目(2005ABA195)

摘　　要：为开发猪日本脑炎新型疫苗,采用PCR方法扩增日本脑炎病毒WHe株prME和NS1基因,并将其分别克隆至DNA疫苗载体pVAX1,分别用酶切和测序分析进行鉴定。结果表明prME和NS1基因大小分别为2.1kb和1.1kb,酶切和测序结果表明,猪日本脑炎DNA疫苗pVAX1-prME和pVAX1-NS1构建成功,为进一步免疫试验奠定了基础。In order to develop a novel vaccine against Japanese encephalitis （JE） in swine, the prME（2.1 kb） gene and NSI（1.1 kb） gene of Japanese encephalitis virus （JEV） strain WHe was amplified by polymerase chain reaction and then the genes were cloned into pVAX1 vector respectively. The recombinant vectors were identified by sequencing. The results showed that the DNA vaccine pVAX1-prME and pVAX1-NS1 against JEV in swine were constructed successfully.

关 键 词：日本脑炎 DNA疫苗 构建 鉴定 

分 类 号：S852.659.5[农业科学—基础兽医学] S852.65[农业科学—兽医学]