木质素脱氢聚合物生物合成的探讨  

Study on Biosynthesis of Dehydrogenation Polymers of Lignin

作　　者：杨海涛[1] 谢益民[1] 

机构地区：[1]山东轻工业学院制浆造纸工程省级重点学科,山东济南250100

出　　处：《林产化学与工业》2008年第1期1-5,共5页Chemistry and Industry of Forest Products

基　　金：国家自然科学基金(20674046,30471365);教育部博士点基金(20020561022);山东省中青年科学家奖励基金(04BS05005)

摘　　要：模拟自然条件,以松柏醇葡萄糖苷为原料,在β-葡萄糖苷酶、葡萄糖氧化酶及过氧化物酶存在下,分别用混合法和滴加法,来合成木质素脱氢聚合物(DHP)。通过凝胶渗透色谱(GPC)、FT-IR、13CNMR与1HNMR分析,发现混合法所得DHP中β-5结构含量略高,而滴加法所得DHP中β-O-4结构含量较高。从GPC分析可以得到,混合法DHP的相对分子质量(Mr)低于滴加法DHP的Mr。从而,说明滴加的方法优于直接混合的方法。证实了β-O-4,β-β,β-5,β-1为DHP中的主要结构单元,此外还含有大量的松柏醇结构、少量香草醛及带有亚甲基结构的苯丙烷结构和以醚键与木质素相结合的少量碳水化合物。Guaiacyl-type dehydrogenation polymers （DHPs） of lignin were prepared from coniferin by bulk method and end-wise method respectively in the presence of peroxidase, glucose oxidase and β-glucosidase in the laboratory to simulate natural condition in plant. The DHPs were analyzed by GPC, FT-IR, ^1H NMR and ^13C NMR. The result of analysis indicated that the chemical structure of DHP from bulk method had higher content of β-5 structure and the chemical structure of DHP from end-wise method had higher content of β-O-4 structure. The DHP from end-wise method exhibited higher relative molecular weight （Mr） than the DHP from bulk method. It was also proved thatβ-O-4,β-β,β-5 and β-1 structures were main substructures in DHPs although there were some minor substructures such as coniferyl alcohol, vanillin, phenylpropane units with a-methylene and some lignin moieties connected with carbohydrate through benzyl ether linkage.

关 键 词：松柏醇葡萄糖苷 木质素脱氢聚合物 混合法 滴加法 

分 类 号：TQ351[化学工程] TS711[轻工技术与工程—制浆造纸工程]