人体上皮细胞系(株)间交叉污染的检测  

DETECTION OF CROSS-CONTAMINATION BETWEEN DIFFERENT HUMAN EPITHELIAL CELL LINES

作　　者：张荣兴[1] 师双平[2] 朱德厚 葛锡锐[1] 

机构地区：[1]中国科学院上海细胞生物学研究所,200031 [2]南京大学生物系

出　　处：《细胞生物学杂志》1997年第2期87-89,共3页Chinese Journal of Cell Biology

基　　金：中国科学院"八五"重点项目基金

摘　　要：本文根据不同上皮细胞的角蛋白丝性质和多肽组成的差异,建立了四种不同上皮细胞系(株)间交叉污染的检测方法:1.SDS-PAGE法;2.免疫印迹法;3.AE1单抗免疫荧光染色法;4.角蛋白丝结构转化法。结果表明:方法1—3比较有用。我们认为,要获得较满意的检测结果,需要根据不同上皮细胞的特点,选择不同的方法配合使用。Based on differences in characteristics and composition of polypeptides of keratin filame-nts in human epithelial cell lines, we established four methods to detect cross-contamination between different epithelial cell lines: 1. SDS-PAGE of keratins, 2. Westem blot with AE 1 monoclonal antibody against keratins, 3. Immunofluorence staining with AE l antibody, and 4. Structural transformation of keratin filaments. The results indicate that methods 1-3 are more useful. In order to get satisfactory detection, it must choose combination of several methods according to the properties of the respective epithelial cells.

关 键 词：细胞生物学 上皮细胞 交叉污染 检测 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]