铝对原代培养大鼠海马神经细胞毒性作用的初步观察  

OBSERVATION ON THE ALUMINIUM'S NEUROTOXICITY ON THE PRIMARY CULTURED RAT HIPPOCAMPAL NEURONS

作　　者：田德全[1] 严振国[1] 

机构地区：[1]上海中医药大学,200032

出　　处：《细胞生物学杂志》1997年第3期136-139,共4页Chinese Journal of Cell Biology

摘　　要：新生一天的大鼠海马神经细胞在含10％胎牛血清和10％小牛血清的DMEM培养液,5％CO_2,37℃的环境中可以获得良好的生长。用从微摩尔浓度至毫摩尔浓度的铝处理细胞1小时,细胞死亡率明显增加,并呈剂量依赖性。但10^(-3)mol/L浓度的铝却使死亡率突然降低,与10^(-4)mol/L比较具有极显著的统计学意义。铝的神经毒作用与细胞外液中钙离子存在与否无关,不能用传统的兴奋性学说来解释。Hippocampal neurons dissected from the one-day newly-born rats can be cultured well in DMEM medium supplemented with 10% fetal calf serum and 10% new-born bovine serum in a 37℃, 5%CO2 incubator. Cell mortality were remarkably increased in a dose-depentent man-ner, when the cell treated for one hour with aluminium of 10-6mol/L, 10-5mol/L, 10-4mol/L concentration respectively. But aluminium of 10-3mol/L caused a significant decline of cell mortality comparing with that of 10-4.urol/L Aluminium neurotoxicity has no relation with the extracellular calcium, which unable to be explained by the traditional excititoxity hypothesis.

关 键 词：铝 毒性 海马 神经细胞 原代培养 神经毒性 

分 类 号：R995[医药卫生—毒理学] R740.2[医药卫生—药学]