肺动脉内皮细胞与肺动脉平滑肌细胞增殖的相互调节  

MUTUAL REGULATION OF PROLIFERATION BETWEEN PULMONARY ARTERY ENDOTHELIAL CELLS AND PULMONARY SMOOTH MUSCLE CELLS IN VITRO

作　　者：王培勇[1,2] 刘健[1,2] 廖维公 许蜀闽[1,2] 罗德成 孙秉庸[1,2] 

机构地区：[1]第三军医大学高原医学研究室病理生理教研室 [2]北京医科大学心血管病基础研究所

出　　处：《中国应用生理学杂志》1997年第3期209-212,共4页Chinese Journal of Applied Physiology

基　　金：自然科学基金

摘　　要：血管内皮细胞和血管平滑肌细胞在结构和功能上关系密切，二者的相互关系在血管舒缩和血管壁结构的调节中起重要作用。本文观察了培养的小牛肺动脉内皮细胞（ＰＡＥＣ）和肺动脉平滑肌细胞（ＰＡＳＭ）在细胞增殖方面的相互调节作用。混合培养的ＰＡＥＣ和ＰＡＳＭ细胞的３Ｈ－ＴｄＲ参入明显降低（Ｐ＜０．００１，与对照组相比）。无论向培养的ＰＡＥＣ和ＰＡＳＭ中分别加入ＰＡＳＭ和ＰＡＥＣ的条件培养基还是二者共培养时，均发现ＰＡＥＣ的３Ｈ－ＴｄＲ参入明显降低，而ＰＡＳＭ的３Ｈ－ＴｄＲ明显升高（Ｐ＜０．０５，与对照组相比）。流式细胞测定也发现共培养时ＰＡＥＣ的Ｇ１期细胞增多，Ｇ２／Ｍ期细胞减少；而ＰＡＳＭ的Ｇ１期细胞减少，Ｇ２／Ｍ期细胞增多。共培养的ＰＡＳＭ细胞内ｃＡＭＰ增加，ｃＧＭＰ含量降低；而ＰＡＥＣ细胞的ｃＡＭＰ和ｃＧＭＰ含量均降低（Ｐ＜０．０１，与对照组相比）。上述结果提示。Vascular endothelial cell are closely related to vascular smooth muscle cells in structure and function. The interactions between them may play important roles in the modulation of function and structure of vascular wall. In the present study, the mitogenic regulations between cultured new bovine pulmonary arterial endothelial cell (PAEC) and pulmonary arterial smooth muscle (PASM) were investigated. When PAECs and PASMs were mixcultured, 3 H TdR incorporation into the mixed cells decreased significantly( P <0.001 vs control) . When PAECs and PASMs were cultured in conditioned medium from PASMs and PAECs respectively or they were cocultured, the proliferation of PAECs was inhibited while that of PASM was stimulated significantly ( P <0.05 vs control). It was also found that the concentration of cAMP increased but cGMP decreased in cocultured PASMs( P <0.01 vs control), while the concentration of both cAMP and cGMP decreased significantly in cocultured PAECs( P <0.01 vs control). These findings suggest that PAECs and PASMs may regulate their proliferation each other through the second messenger system.

关 键 词：肺动脉 内皮细胞 平滑肌细胞 增殖 肺动脉高压 

分 类 号：R331.33[医药卫生—人体生理学] R543.202[医药卫生—基础医学]