rhCNTF对鸡胚感觉与运动神经元神经营养作用的比较  

COMPARISON BETWEEN THE EFFECTS OF rhCNTF ON SENSORY AND MOTOR NEURON OF CHICKEN EMBRYO

作　　者：咸海青[1,2] 刘淑红[1,2] 顾晓松[1,2] 严志强[1,2] 范明[1,2] 

机构地区：[1]军事医学科学院基础医学研究所 [2]南通医学院神经科学研究所

出　　处：《中国应用生理学杂志》1997年第3期268-270,共3页Chinese Journal of Applied Physiology

摘　　要：在无血清培养条件下，观察了重组人睫状营养因子（ｒｈＣＮＴＦ）对鸡胚背根节感觉神经元及腹角运动神经元的营养作用。结果表明ｒｈＣＮＴＦ对这两类神经元均有明显的促存活作用，并呈一定的剂量／效应关系。ｒｈＣＮＴＦ浓度在０．５ｎｇ／ｍｌ以下时无作用，１．０－１．５ｎｇ／ｍｌ时已有促神经元存活作用，４ｎｇ／ｍｌ时作用最明显，再增加到１００ｎｇ／ｍｌ神经元存活数无进一步增加。比较培养７天时两类神经元存活数发现感觉神经元对ＣＮＴＦ缺乏的敏感性高于运动神经元。The effects of rhCNTF on dorsal root ganglia (DRG) sensory neurons and spinal cord (SC) motor neurons of 10 day chicken embryos were observed with serum free culture. The results indicated that rhCNTF supported the survival and differentiation of these neurons and showed certain dose dependent manners. No effect was found at 0 5ng/ml rhCNTF concentration, a little survival promotive effect at 1 0-1 5ng/ml, peak effect at 4ng/ml and no more effects from 8 0 to 100ng/ml. The survival number of neurons on 7th day was compared, and results showed higher sensitivity of sensory neuron to CNTF shortage than that of motoneuron. This suggests that survival effect of CNTF on motoneuron is only small part of its neurotrophic effects.

关 键 词：rhCNTF 运动神经元 感觉神经元 神经营养作用 

分 类 号：R338.1[医药卫生—人体生理学] Q516[医药卫生—基础医学]