一种简易的小动物一氧化氮吸入系统的研制  

A SIMPLE NITRIC OXIDE EXPOSURE SYSTEM FOR SMALL ANIMALS

作　　者：熊长明 程显声[1,2] 何建国[1,2] 崔希忠 李智[1,2] 

机构地区：[1]中国医学科学院中国协和医科大学心血管病研究所 [2]阜外心血管病医院

出　　处：《中国应用生理学杂志》1997年第3期275-277,共3页Chinese Journal of Applied Physiology

基　　金：卫生部科学基金

摘　　要：作者自行设计了一套小动物一氧化氮（ＮｉｔｒｉｃＯｘｉｄｅ，ＮＯ）吸入系统，该系统主要包括有机玻璃舱、采气泵、流量计、ＮＯ／Ｎ２混和气体、纯氧、氮氧化物分析仪、Ｏ２、ＣＯ２监测仪。实验过程中，将６－７只大鼠置于舱内分别吸入２０ｐｐｍ、４０ｐｐｍ、８０ｐｐｍ的ＮＯ８ｈ，每２０ｍｉｎ记录一次舱内ＮＯ、ＮＯ２、Ｏ２和ＣＯ２的浓度值。结果表明舱内的实测ＮＯ浓度与实验所设计的ＮＯ浓度相近，舱内氧浓度与空气中的氧浓度相似，ＮＯ２和ＣＯ２最大浓度分别不超过３ｐｐｍ和０．３％，而且在整个吸入过程中，舱内各气体浓度趋于稳定。该系统结构简单，使用方便，可用于大鼠长期吸入ＮＯ的实验研究作者自行设计了一套小动物一氧化氮（ＮｉｔｒｉｃＯｘｉｄｅ，ＮＯ）吸入系统，该系统主要包括有机玻璃舱、采气泵、流量计、ＮＯ／Ｎ２混和气体、纯氧、氮氧化物分析仪、Ｏ２、ＣＯ２监测仪。实验过程中，将６－７只大鼠置于舱内分别吸入２０ｐｐｍ、４０ｐｐｍ、８０ｐｐｍ的ＮＯ８ｈ，每２０ｍｉｎ记录一次舱内ＮＯ、ＮＯ２、Ｏ２和ＣＯ２的浓度值。结果表明舱内的实测ＮＯ浓度与实验所设计的ＮＯ浓度相近，舱内氧浓度与空气中的氧浓度相似，ＮＯ２和ＣＯ２最大浓度分别不超过３ｐｐｍ和?A nitric oxide exposing system was designed for experimental research, which consist of equipments such as plexiglass chamber, blower, flowmeter, NO/N 2 cylinder, pure O 2 cyclinder, NO x analyzer, and O 2/CO 2 monitor. The efficacy of the whole system has been verified through our practice as shown by the following results: measured NO inside the chamber were close to designed NO concentrations, measured O 2 concentrations in the chamber were similar to that of the atmosphere, and the highest nitrogen dioxide(NO 2) and carbon dioxide(CO 2) concentrations were lower than 3ppm and 0 3% respectively. The experimental facility is simple in construction, easy to be operated and convenient for research on effects and toxicity of long term inhaled nitric oxide in small animals.

关 键 词：一氧化氮 吸入系统 小动物 实验装置 

分 类 号：Q95－337[生物学—动物学]